Entrainment of cerebellar Purkinje cell spiking activity using pulsed ultrasound stimulation.
Entrainment of cerebellar Purkinje cell spiking activity using pulsed ultrasound stimulation.
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DOI：
10.1016/j.brs.2021.03.004
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发表时间：
2021-05
影响因子：
7.7
通讯作者：
Sahin M
中科院分区：
文献类型：
--
作者：
Asan AS;Kang Q;Oralkan Ö;Sahin M
聚焦超声（FUS）在空间分辨率和目标操纵能力方面具有优于其他非侵入性刺激方法的特性。FUS尚未在小脑皮质中进行测试，FUS的细胞效应尚未完全了解。探讨不同脉冲宽度和重复频率的FUS对小脑浦肯野细胞（PC）活动的影响。将玻璃微电极从背侧插入小脑蚓部第6小叶，在细胞外记录PCs的单个单位活动。通过填充有脱气水的耦合锥从后侧施加超声刺激（500 kHz），以不同的脉冲持续时间和频率靶向记录区域。简单的尖峰（SS）活动的PC夹带的FUS模式，其中尖峰发生的概率峰值在1毫秒左右的刺激开始后，无论其持续时间（0.5，1，或2毫秒）。在50 Hz脉冲重复频率（PRF）下，脉冲持续时间越短，夹带水平越强，然而，在100 Hz PRF下，事件周围直方图分布越宽，峰值略微延迟，表明涉及持久的抑制机制。基线和刺激期的平均放电率之间无显著差异。FUS可以在一个尖峰的基础上夹带单细胞的尖峰活动，如这里在大鼠小脑皮质中所证明的。所观察到的调制可能是由于FUS对整个皮层网络的兴奋和抑制作用的聚集，而不是单独对PC的。
Focused ultrasound (FUS) has excellent characteristics over other non-invasive stimulation methods in terms of spatial resolution and steering capability of the target. FUS has not been tested in the cerebellar cortex and cellular effects of FUS are not fully understood. To investigate how the activity of cerebellar Purkinje cells (PCs) is modulated by FUS with varying pulse durations and pulse repetition frequencies. A glass microelectrode was inserted into the cerebellar vermis lobule 6 from the dorsal side to extracellularly record single unit activity of the PCs in anesthetized rats. Ultrasonic stimulation (500 kHz) was applied through a coupling cone, filled with degassed water, from the posterior side to target the recording area with varying pulse durations and frequencies. Simple spike (SS) activity of PCs was entrained by the FUS pattern where the probability of spike occurrences peaked at around 1 ms following the onset of the stimulus regardless of its duration (0.5, 1, or 2 ms). The level of entrainment was stronger with shorter pulse durations at 50-Hz pulse repetition frequency (PRF), however, peri-event histograms spread wider and the peaks delayed slightly at 100-Hz PRF, suggesting involvement of a long-lasting inhibitory mechanism. There was no significant difference between the average firing rates in the baseline and stimulation periods. FUS can entrain spiking activity of single cells on a spike-by-spike basis as demonstrated here in the rat cerebellar cortex. The observed modulation potentially results from the aggregate of excitatory and inhibitory effects of FUS on the entire cortical network rather than on the PCs alone.
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