DNA double-strand breaks alter the spatial arrangement of homologous loci in plant cells.
DNA double-strand breaks alter the spatial arrangement of homologous loci in plant cells.
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DOI：
10.1038/srep11058
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发表时间：
2015-06-05
影响因子：
4.6
通讯作者：
Matsunaga S
中科院分区：
文献类型：
--
作者：
Hirakawa T;Katagiri Y;Ando T;Matsunaga S
染色质动力学和排列涉及真核生物（包括植物）细胞核中的许多生物过程。植物不能移动，因此必须对各种环境刺激做出快速反应才能实现生长和发育。假设染色质动力学和排列的改变支持对这些刺激的反应；然而，植物中的信息很少。在本研究中，我们通过 lacO/LacI-EGFP 系统的活细胞成像和模拟分析，研究了拟南芥中 DNA 损伤的染色质动态和排列。结果表明，在一般生长过程中，拟南芥根的细胞核中同源位点保持恒定的距离。我们还发现，DNA 双链断裂 (DSB) 会通过 γ 辐射诱导同源位点接近。此外，AtRAD54在同源重组修复途径中发挥重要作用，参与γ-辐射同源位点的配对。这些结果表明同源基因座彼此接近以修复 DSB，而 AtRAD54 介导了这些现象。
Chromatin dynamics and arrangement are involved in many biological processes in nuclei of eukaryotes including plants. Plants have to respond rapidly to various environmental stimuli to achieve growth and development because they cannot move. It is assumed that the alteration of chromatin dynamics and arrangement support the response to these stimuli; however, there is little information in plants. In this study, we investigated the chromatin dynamics and arrangement with DNA damage in Arabidopsis thaliana by live-cell imaging with the lacO/LacI-EGFP system and simulation analysis. It was revealed that homologous loci kept a constant distance in nuclei of A. thaliana roots in general growth. We also found that DNA double-strand breaks (DSBs) induce the approach of the homologous loci with γ-irradiation. Furthermore, AtRAD54, which performs an important role in the homologous recombination repair pathway, was involved in the pairing of homologous loci with γ-irradiation. These results suggest that homologous loci approach each other to repair DSBs, and AtRAD54 mediates these phenomena.
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影响因子：
4.6
作者：
Hayashi, Kohma;Hasegawa, Junko;Matsunaga, Sachihiro
通讯作者：
Matsunaga, Sachihiro
Condensin II亚基DCAP-D3限制了果蝇体细胞中的逆转录子动员。
影响因子：
4.5
作者：
Schuster AT;Sarvepalli K;Murphy EA;Longworth MS
通讯作者：
Longworth MS
DOI：
10.1083/jcb.201208008
发表时间：
2013-02-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ono T;Yamashita D;Hirano T
通讯作者：
Hirano T
影响因子：
16
作者：
Grob, Stefan;Schmid, Marc W.;Grossniklaus, Ueli
通讯作者：
Grossniklaus, Ueli
影响因子：
64.5
作者：
Kitamura E;Blow JJ;Tanaka TU
通讯作者：
Tanaka TU