Interaction of protein phosphatase 1δ with nucleophosmin in human osteoblastic cells.
Interaction of protein phosphatase 1δ with nucleophosmin in human osteoblastic cells.
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DOI：
10.1267/ahc.11041
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发表时间：
2012-02-29
影响因子：
2.4
通讯作者：
Morimoto H
中科院分区：
文献类型：
--
作者：
Haneji T;Teramachi J;Hirashima K;Kimura K;Morimoto H
蛋白质磷酸化和去磷酸化已被认为是调节各种组织中细胞代谢和功能的重要机制。丝氨酸和苏氨酸蛋白磷酸酶（PP）分为四类：PP1、PP2A、PP2B 和 PP2C。在大鼠中分离出至少四种 PP1 催化亚基亚型：PP1α、PP1γ1、PP1γ2 和 PP1δ。在本研究中，我们检查了 PP1δ 在人成骨 Saos-2 细胞中的定位和表达。抗 PP1δ 抗体可识别 Saos-2 细胞核仁区域中存在的蛋白质。细胞分级分离显示 PP1δ 是一种位于核仁中的 37 kDa 蛋白质。核磷蛋白是一种核仁磷蛋白，主要位于核仁中。 Saos-2细胞中核磷蛋白的染色模式与PP1δ相似。 PP1δ 和核磷蛋白在细胞核中被特异地染色为点。双荧光图像显示 PP1δ 和核磷蛋白位于核仁的相同区域。在成骨细胞 MG63 中观察到 PP1δ 和核磷蛋白的相似分布模式。免疫沉淀和 Western 分析也显示了 PP1δ 和核磷蛋白的相互作用。这些结果表明，PP1δ 直接与核仁中的核磷蛋白结合，并表明核磷蛋白是 PP1δ 的候选底物之一。
Protein phosphorylation and dephosphorylation has been recognized as an essential mechanism in the regulation of cellular metabolism and function in various tissues. Serine and threonine protein phosphatases (PP) are divided into four categories: PP1, PP2A, PP2B, and PP2C. At least four isoforms of PP1 catalytic subunit in rat, PP1α, PP1γ1, PP1γ2, and PP1δ, were isolated. In the present study, we examined the localization and expression of PP1δ in human osteoblastic Saos-2 cells. Anti-PP1δ antibody recognized a protein present in the nucleolar regions in Saos-2 cells. Cellular fractionation revealed that PP1δ is a 37 kDa protein localized in the nucleolus. Nucleophosmin is a nucleolar phosphoprotein and located mainly in the nucleolus. Staining pattern of nucleophosmin in Saos-2 cells was similar to that of PP1δ. PP1δ and nucleophosmin were specifically stained as dots in the nucleus. Dual fluorescence images revealed that PP1δ and nucleophosmin were localized in the same regions in the nucleolus. Similar distribution patterns of PP1δ and nucleophosmin were observed in osteoblastic MG63 cells. The interaction of PP1δ and nucleophosmin was also shown by immunoprecipitation and Western analysis. These results indicated that PP1δ associate with nucleophosmin directly in the nucleolus and suggested that nucleophosmin is one of the candidate substrate for PP1δ.
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影响因子：
4.1
作者：
Murányi, A;Erdódi, F;Hartshorne, DJ
通讯作者：
Hartshorne, DJ
影响因子：
2.4
作者：
Noda, Yuya;Amano, Ikuko;Sawa, Yoshihiko
通讯作者：
Sawa, Yoshihiko
影响因子：
2.4
作者：
Hata, Minoru;Amano, Ikuko;Sawa, Yoshihiko
通讯作者：
Sawa, Yoshihiko
影响因子：
13.8
作者：
Bollen, Mathieu;Peti, Wolfgang;Ragusa, Michael J.;Beullens, Monique
通讯作者：
Beullens, Monique
DOI：
10.1111/j.1349-7006.1990.tb02690.x
发表时间：
1990-12
期刊：
Japanese journal of cancer research : Gann
影响因子：
--
作者：
Sasaki K;Shima H;Kitagawa Y;Irino S;Sugimura T;Nagao M
通讯作者：
Nagao M