Fine regulation of ARF17 for anther development and pollen formation.
Fine regulation of ARF17 for anther development and pollen formation.
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ARF17 对花药发育和花粉形成的精细调控
DOI：
10.1186/s12870-017-1185-1
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发表时间：
2017-12-19
影响因子：
5.3
通讯作者：
Yang ZN
中科院分区：
文献类型：
--
作者：
Wang B;Xue JS;Yu YH;Liu SQ;Zhang JX;Yao XZ;Liu ZX;Xu XF;Yang ZN
背景在拟南芥中，绒毡层和小孢子母细胞对于花粉形成至关重要。先前的研究表明，ARF17在小孢子母细胞和四分体中表达，并直接调节花粉形成的四分体壁合成。ARF17是miR160的直接靶标，启动子ARF17::5mARF17(5mARF17/WT)转基因植物在miR160互补域内有5个沉默突变，是不育的。结果在这里，我们发现ARF17也在小孢子母细胞和四分体中表达。绒毡层，在arf17突变体中存在缺陷。与arf17突变体相比,5mARF17/WT植株绒毡层细胞和四分体异常,但绒毡层空泡较少。免疫细胞化学分析表明，ARF17蛋白在花药早期在5mARF17/WT植株的绒毡层、小孢子母细胞和四分体中过度积累，但其表达模式在花药发育过程中不受影响。5mARF17由其天然启动子驱动并不能挽救thearf17雄性不育表型。由绒毡层特异性启动子A9驱动的5mARF17的表达导致转基因植物中绒毡层缺陷和雄性不育。这些结果表明，5mARF17/WT植物的绒毡层和小孢子母细胞中ARF17的过度表达导致雄性不育。微阵列数据显示，大量参与转录和翻译的基因在5mARF17/WT植物中异位表达。结论我们的工作表明，ARF17在花药发育和花粉形成中发挥重要作用，并且miR160调控下的ARF17表达对其在花药发育过程中的功能至关重要。
BackgroundInArabidopsis, the tapetum and microsporocytes are critical for pollen formation. Previous studies have shown thatARF17is expressed in microsporocytes and tetrads and directly regulates tetrad wall synthesis for pollen formation.ARF17is the direct target ofmiR160, andpromoterARF17::5mARF17(5mARF17/WT) transgenic plants, which have five silent mutations within themiR160-complementary domain, are sterile.ResultsHere, we found that ARF17 is also expressed in the tapetum, which was defective inarf17mutants. Compared witharf17mutants,5mARF17/WT plants had abnormal tapetal cells and tetrads but were less vacuolated in the tapetum. Immunocytochemical assays showed that the ARF17 protein over-accumulated in tapetum, microsporocytes and tetrads of5mARF17/WT plants at early anther stages, but its expression pattern was not affected during anther development.5mARF17driven by its native promoter did not rescue thearf17male-sterile phenotype. The expression of5mARF17driven by the tapetum-specific promoterA9led to a defective tapetum and male sterility in transgenic plants. These results suggest that the overexpression of ARF17 in the tapetum and microsporocytes of5mARF17/WT plants leads to male sterility. Microarray data revealed that an abundance of genes involved in transcription and translation are ectopically expressed in5mARF17/WT plants.ConclusionsOur work shows that ARF17 plays an essential role in anther development and pollen formation, and ARF17 expression undermiR160regulation is critical for its function during anther development.
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影响因子：
4.5
作者：
Bruex A;Kainkaryam RM;Wieckowski Y;Kang YH;Bernhardt C;Xia Y;Zheng X;Wang JY;Lee MM;Benfey P;Woolf PJ;Schiefelbein J
通讯作者：
Schiefelbein J
影响因子：
3.7
作者：
Shi ZH;Zhang C;Xu XF;Zhu J;Zhou Q;Ma LJ;Niu J;Yang ZN
通讯作者：
Yang ZN
DOI：
10.1073/pnas.0908718106
发表时间：
2009-12-29
影响因子：
11.1
作者：
Nag, Anwesha;King, Stacey;Jack, Thomas
通讯作者：
Jack, Thomas
影响因子：
10.5
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
通讯作者：
Zhao, Yunde
影响因子：
11.6
作者：
Wang, JW;Wang, LJ;Chen, XY
通讯作者：
Chen, XY