Unexpected invasion of miniature inverted-repeat transposable elements in viral genomes.
Unexpected invasion of miniature inverted-repeat transposable elements in viral genomes.
复制标题
病毒基因组中微型反向重复转座元件的意外入侵
DOI：
10.1186/s13100-018-0125-4
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发表时间：
2018
期刊：
影响因子：
4.9
通讯作者：
Dai FY
中科院分区：
文献类型：
--
作者：
Zhang HH;Zhou QZ;Wang PL;Xiong XM;Luchetti A;Raoult D;Levasseur A;Santini S;Abergel C;Legendre M;Drezen JM;Béliveau C;Cusson M;Jiang SH;Bao HO;Sun C;Bureau TE;Cheng PF;Han MJ;Zhang Z;Zhang XG;Dai FY
背景转座元件(TES)在细胞基因组中十分常见，通常具有很高的拷贝数。与细胞生物不同，TES以前被认为在病毒中要么罕见，要么不存在。几乎所有报道的TES都显示每个病毒基因组只有一到两个拷贝。此外，基因组高达2.5-Mb的大病毒的发现强调了生物学家重新思考病毒与细胞生命之间关系的根本性质的必要性。结果在此，我们首次对现有序列的5170株病毒基因组中的微型反向重复转座元件(MET)进行了全面的分析。在病毒中发现了10个微型反向重复转座元件的451个拷贝，每个粉尘都达到了相对较大的拷贝数(有些高达90个)。首次在病毒中发现了属于两个DNA超家族(HOBO/ACTIVATOR/TAM3和Chapaev-MIRAGE-CACTA)的八种螨类，进一步扩大了这两个超家族的生物范围。TES可能在塑造大病毒基因组的进化过程中发挥重要作用，这里发现的大病毒基因组中含有非常丰富的螨类。我们还发现，在病毒宿主的基因组中存在七种螨类的假定自主伙伴，这表明病毒可能借用其细胞宿主自主元件的转座机制来传播螨类并定植自己的基因组。在病毒宿主中存在7种相似的螨类，这表明水平转移(HTS)是螨类传播的主要机制。结论我们的发现强调了TES对大流行病毒基因组进化的影响。我们得出的结论是，对于细胞生物来说，TES是大流行病毒多样化移动体的一部分。
BackgroundTransposable elements (TEs) are common and often present with high copy numbers in cellular genomes. Unlike in cellular organisms, TEs were previously thought to be either rare or absent in viruses. Almost all reported TEs display only one or two copies per viral genome. In addition, the discovery of pandoraviruses with genomes up to 2.5-Mb emphasizes the need for biologists to rethink the fundamental nature of the relationship between viruses and cellular life.ResultsHerein, we performed the first comprehensive analysis of miniature inverted-repeat transposable elements (MITEs) in the 5170 viral genomes for which sequences are currently available. Four hundred and fifty one copies of ten miniature inverted-repeat transposable elements (MITEs) were found and each MITE had reached relatively large copy numbers (some up to 90) in viruses. Eight MITEs belonging to two DNA superfamilies (hobo/Activator/Tam3andChapaev–Mirage–CACTA) were for the first time identified in viruses, further expanding the organismal range of these two superfamilies. TEs may play important roles in shaping the evolution of pandoravirus genomes, which were here found to be very rich in MITEs. We also show that putative autonomous partners of seven MITEs are present in the genomes of viral hosts, suggesting that viruses may borrow the transpositional machinery of their cellular hosts’ autonomous elements to spread MITEs and colonize their own genomes. The presence of seven similar MITEs in viral hosts, suggesting horizontal transfers (HTs) as the major mechanism for MITEs propagation.ConclusionsOur discovery highlights that TEs contribute to shape genome evolution of pandoraviruses. We concluded that as for cellular organisms, TEs are part of the pandoraviruses’ diverse mobilome.
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影响因子：
14.9
作者：
Clark KJ;Carlson DF;Leaver MJ;Foster LK;Fahrenkrug SC
通讯作者：
Fahrenkrug SC
影响因子：
1.5
作者：
Dupuy, C.;Periquet, G.;Drezen, J-M
通讯作者：
Drezen, J-M
影响因子：
16.6
作者：
Gilbert, Clement;Chateigner, Aurelien;Ernenwein, Lise;Barbe, Valerie;Bezier, Annie;Herniou, Elisabeth A.;Cordaux, Richard
通讯作者：
Cordaux, Richard
影响因子：
3.8
作者：
Djoumad, Abdelmadjid;Stoltz, Don;Cusson, Michel
通讯作者：
Cusson, Michel
影响因子：
4.6
作者：
Filee, Jonathan;Chandler, Michael
通讯作者：
Chandler, Michael