First report of a thioredoxin homologue in jellyfish: molecular cloning, expression and antioxidant activity of CcTrx1 from Cyanea capillata.
First report of a thioredoxin homologue in jellyfish: molecular cloning, expression and antioxidant activity of CcTrx1 from Cyanea capillata.
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水母硫氧还蛋白同源物的首次报道：Cyanea capillata CcTrx1 的分子克隆、表达和抗氧化活性
DOI：
10.1371/journal.pone.0097509
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Ruan Z;Liu G;Guo Y;Zhou Y;Wang Q;Chang Y;Wang B;Zheng J;Zhang L
硫氧还蛋白（Thioredoxins，Trx）是一类广泛存在的高度保守的小分子蛋白，在抗氧化损伤中发挥重要作用。在本研究中，从水母Cyanea capillata的触角cDNA文库中鉴定了Trx的同源物，命名为CcTrx 1。CcTrx 1全长cDNA为479 bp，开放阅读框为312 bp，编码104个氨基酸。生物信息学分析表明，CcTrx1蛋白具有进化上保守的Trx活性位点31CGPC34，与其他生物的Trx1蛋白具有高度的相似性，表明CcTrx1是Trx1亚家族的一个新成员。CcTrx1 mRNA在触角、伞、口臂和性腺中组成型表达，表明CcTrx1蛋白在各种生理过程中具有普遍作用。在大肠杆菌BL21（DE3）中表达重组CcTrx1（rCcTrx1）蛋白，并经亲和层析纯化。rCcTrx1蛋白被证明具有预期的氧化还原活性，在酶分析和保护超螺旋DNA的氧化损伤。这些结果表明，CcTrx1可能是C.毛状的据我们所知，这是第一个Trx蛋白的特点，从水母物种。
Thioredoxins (Trx proteins) are a family of small, highly-conserved and ubiquitous proteins that play significant roles in the resistance of oxidative damage. In this study, a homologue of Trx was identified from the cDNA library of tentacle of the jellyfish Cyanea capillata and named CcTrx1. The full-length cDNA of CcTrx1 was 479 bp with a 312 bp open reading frame encoding 104 amino acids. Bioinformatics analysis revealed that the putative CcTrx1 protein harbored the evolutionarily-conserved Trx active site 31CGPC34 and shared a high similarity with Trx1 proteins from other organisms analyzed, indicating that CcTrx1 is a new member of Trx1 sub-family. CcTrx1 mRNA was found to be constitutively expressed in tentacle, umbrella, oral arm and gonad, indicating a general role of CcTrx1 protein in various physiological processes. The recombinant CcTrx1 (rCcTrx1) protein was expressed in Escherichia coli BL21 (DE3), and then purified by affinity chromatography. The rCcTrx1 protein was demonstrated to possess the expected redox activity in enzymatic analysis and protection against oxidative damage of supercoiled DNA. These results indicate that CcTrx1 may function as an important antioxidant in C. capillata. To our knowledge, this is the first Trx protein characterized from jellyfish species.
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影响因子：
7.4
作者：
Circu, Magdalena L.;Aw, Tak Yee
通讯作者：
Aw, Tak Yee
DOI：
10.1016/j.cbpb.2007.12.009
发表时间：
2008-04-01
影响因子：
2.2
作者：
De Zoysa, Mahanama;Pushpamali, Wickramaarachchilage Anoja;Lee, Jehee
通讯作者：
Lee, Jehee
影响因子：
3.7
作者：
Hoflehner E;Binder M;Hemmer W;Mahler V;Panzani RC;Jarisch R;Wiedermann U;Duchêne M
通讯作者：
Duchêne M
影响因子：
5.8
作者：
Arnold, K;Bordoli, L;Schwede, T
通讯作者：
Schwede, T
影响因子：
56.9
作者：
FRIDOVICH, I
通讯作者：
FRIDOVICH, I