Structure and function of the bacterial root microbiota in wild and domesticated barley.
Structure and function of the bacterial root microbiota in wild and domesticated barley.
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DOI：
10.1016/j.chom.2015.01.011
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发表时间：
2015-03-11
影响因子：
30.3
通讯作者：
Schulze-Lefert P
中科院分区：
文献类型：
--
作者：
Bulgarelli D;Garrido-Oter R;Münch PC;Weiman A;Dröge J;Pan Y;McHardy AC;Schulze-Lefert P
微生物群落栖息在健康植物的根内部，以及根际，其中包括土壤颗粒牢固地附着在根上，与它们的宿主进行共生。为了研究这些社区之间的结构和功能的多样性，我们采用了16 S rRNA基因分析和鸟枪宏基因组分析与野生和驯化的大麦（大麦）加入相关的微生物群的组合。细菌科丛枝孢菌科、黄杆菌科和根瘤菌科在大麦根富集的微生物群中占主导地位。宿主基因型对根相关细菌群落的多样性有一个小的，但显着的影响，可能代表了大麦驯化的足迹。与发病机制，分泌，噬菌体相互作用和营养动员相关的性状在大麦根相关微生物群中富集。引人注目的是，分配给这些相同性状的蛋白质家族显示出正选择的证据。我们的研究结果表明，微生物-微生物和宿主-微生物相互作用的联合作用驱动了根-土界面微生物区系的分化。一小部分细菌家族在根富集的大麦微生物群中占主导地位宿主基因型决定了群落成员的一个子集的概况与宿主相互作用相关的功能在根相关的类群中富集介导宿主、细菌和噬菌体相互作用的基因显示出正选择的迹象栖息在根内部和周围土壤中的微生物群落有助于植物生长。Bulgilli等人研究微生物群，人口的根大麦（大麦）和目前的证据表明，微生物-微生物和宿主-微生物相互作用的综合行动驱动根微生物群的建立，通过发生在根-土界面的生理过程。
The microbial communities inhabiting the root interior of healthy plants, as well as the rhizosphere, which consists of soil particles firmly attached to roots, engage in symbiotic associations with their host. To investigate the structural and functional diversification among these communities, we employed a combination of 16S rRNA gene profiling and shotgun metagenome analysis of the microbiota associated with wild and domesticated accessions of barley (Hordeum vulgare). Bacterial families Comamonadaceae, Flavobacteriaceae, and Rhizobiaceae dominate the barley root-enriched microbiota. Host genotype has a small, but significant, effect on the diversity of root-associated bacterial communities, possibly representing a footprint of barley domestication. Traits related to pathogenesis, secretion, phage interactions, and nutrient mobilization are enriched in the barley root-associated microbiota. Strikingly, protein families assigned to these same traits showed evidence of positive selection. Our results indicate that the combined action of microbe-microbe and host-microbe interactions drives microbiota differentiation at the root-soil interface. A small number of bacterial families dominate the root-enriched barley microbiota The host genotype determines the profile of a subset of community members Functions relevant for host interactions are enriched in root-associated taxa Genes mediating host, bacteria, and phage interactions show signs of positive selection Microbial communities inhabiting the root interior and surrounding soil contribute to plant growth. Bulgarelli et al. examine the microbiota that populates the roots of barley (Hordeum vulgare) and present evidence that integrated actions of microbe-microbe and host-microbe interactions drive root microbiota establishment through physiological processes occurring at the root-soil interface.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
14.9
作者：
Haft DH;Selengut JD;Richter RA;Harkins D;Basu MK;Beck E
通讯作者：
Beck E
影响因子：
4.8
作者：
Clark, JS
通讯作者：
Clark, JS
影响因子：
1.9
作者：
KISLEV, ME;NADEL, D;CARMI, I
通讯作者：
CARMI, I
影响因子：
3.6
作者：
Chelius, MK;Triplett, EW
通讯作者：
Triplett, EW