Lidocaine potentiates the chondrotoxicity of methylprednisolone.
Lidocaine potentiates the chondrotoxicity of methylprednisolone.
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DOI：
10.1016/j.arthro.2009.01.003
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发表时间：
2009-04
期刊：
影响因子：
--
通讯作者：
Chu CR
中科院分区：
文献类型：
--
作者：
Seshadri V;Coyle CH;Chu CR
本研究在模拟炎症环境中检测了甲基强的松龙、甲基强的松龙加利多卡因和甲基强的松龙对牛关节软骨细胞的影响。将牛关节软骨细胞悬浮于藻酸盐微珠中，在Dulbecco‘s改良Eagle’s培养液/F-12中培养1周。将悬浮软骨细胞置于生理盐水(阴性对照)、甲基强的松龙(4、8、16 mg/m L)、甲基强的松龙(8 mg/m L)加1%利多卡因、或甲基强的松龙(8 m g/m L)和生理盐水的模拟炎症环境中(白介素1β暴露，10 ng/m L)15、30和60 m in。分别在暴露后1天、4天和7天用膜联蛋白V和碘化丙啶进行流式细胞术检测软骨细胞活性。4、8和16 mg/mL甲基强的松龙作用60min后，软骨细胞存活率分别从生理盐水中的84%下降到62%、38%和2.4%(n=7，P<0.05)。软骨毒性随着甲基强的松龙暴露时间的延长和暴露后时间的延长而增加。在IL-1β激活的软骨细胞中，甲基强的松龙(8 mg/mL)作用60分钟后，细胞存活率从生理盐水中的76%下降到2.9%(n=4，P<0.05)。8 mg/mL甲基强的松龙和1%利多卡因联合作用60分钟后，细胞存活率进一步下降至1.0%(n=4，P<；0.05)。这些结果表明，在暴露于临床相关剂量的甲基强的松龙后，软骨细胞活力呈剂量和时间依赖性的下降。甲基强的松龙和利多卡因的组合是有毒的，治疗后几乎没有细胞存活。此外，甲基强的松龙并没有减轻IL-1β的炎症效应，相反，它进一步增强了软骨毒性。关节内注射糖皮质激素和局麻药在临床上被广泛使用。这项体外研究提供了这些药物对关节软骨的潜在影响的信息。
This study examined the viability of bovine articular chondrocytes after exposure to methylprednisolone, methylprednisolone with lidocaine, and methylprednisolone in a simulated inflammatory environment. Bovine articular chondrocytes were suspended in alginate beads and cultured in Dulbecco’s modified Eagle’s medium/F-12 for 1 week before experimentation. Suspended chondrocytes were exposed to 0.9% saline solution (negative control), methylprednisolone (4, 8, and 16 mg/mL), methylprednisolone (8 mg/mL) with 1% lidocaine, or methylprednisolone (8 mg/mL) and saline solution in a simulated inflammatory environment (interleukin [IL] 1β exposure, 10 ng/mL) for 15, 30, and 60 minutes. Flow cytometry was performed 1 day, 4 days, and 7 days after exposure by use of annexin V and propidium iodide to assess chondrocyte viability. Chondrocyte viability decreased from 84% in saline solution to 62%, 38%, and 2.4% 1 day after 60 minutes of exposure to 4, 8, and 16 mg/mL of methylprednisolone, respectively (n = 7, P < .05). Chondrotoxicity increased with increasing time of exposure to methylprednisolone and with increasing time after exposure. In IL-1β–activated chondrocytes, viability decreased from 76% in saline solution to 2.9% after 60 minutes of methylprednisolone exposure (8 mg/mL) (n = 4, P < .05). The combination of 8 mg/mL of methylprednisolone and 1% lidocaine further reduced viability to 1.0% after 60 minutes (n = 4, P < .05). These results show a dose- and time-dependent decrease in chondrocyte viability after exposure to clinically relevant doses of methylprednisolone. The combination of methylprednisolone and lidocaine was toxic, with virtually no cells surviving after treatment. In addition, methylprednisolone did not mitigate the inflammatory effects of IL-1β; rather, it further potentiated the chondrotoxicity. Intra-articular injections of corticosteroids and local anesthetics are widely used in clinical practice. This in vitro study provides information on the potential effects of these drugs on articular cartilage.
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影响因子：
5
作者：
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