Avocado fruit maturation and ripening: dynamics of aliphatic acetogenins and lipidomic profiles from mesocarp, idioblasts and seed.
Avocado fruit maturation and ripening: dynamics of aliphatic acetogenins and lipidomic profiles from mesocarp, idioblasts and seed.
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DOI：
10.1186/s12870-017-1103-6
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发表时间：
2017-09-29
影响因子：
5.3
通讯作者：
Díaz de la Garza RI
中科院分区：
文献类型：
--
作者：
Rodríguez-López CE;Hernández-Brenes C;Treviño V;Díaz de la Garza RI
鳄梨果实含有脂肪族乙酸配基（常乙酰化的奇数链脂肪醇），具有良好的生物活性，可用于医疗和食品工业。然而，我们对它们的新陈代谢知之甚少。本工作的目的是研究在果实发育，发芽，和三个收获年的中果皮，含脂异细胞和种子从'哈斯'品种的乙酸根配置文件的变化。还进行了基于非靶向LC-MS的脂质组学分析，以分析每个组织中鳄梨果实的脂质组。分析结果表明，在不同采收年限和采收后成熟、萌发过程中，鳄梨中果皮中的丙酮酸苷成分和含量基本保持不变。然而，在种子中观察到在早期成熟过程中，伴随着浓度的急剧增加，在乙内酯分布的转变。非靶向脂质组学表明，这种转变伴随着甘油脂质的重塑：种子中果实生长期间TAG和DAG减少。值得注意的是，大多数成熟种子中的脂质体由acetogenins组成，我们认为，该组织能够独立地从中果皮合成它们。另一方面，含脂质的异细胞积累了几乎整个中果皮中测得的整个乙内酯池，而只有4%的总脂肪酸。采后果实成熟时，该类型细胞的脂质体变化最大，TAG减少，而奇链DAG增加。值得注意的是，异胚细胞脂质体比中果皮更多样化。这些证据表明，异细胞是鳄梨中果皮中乙酮合成的主要场所。这项工作揭示了鳄梨果实脂质体中脂肪族乙酸配基的普遍性，并证明TAG在其生物合成中作为乙酸配基骨架的初始供体。它还为将来旨在表征鳄梨种子的工作中包括乙酸配基提供了证据，因为它们是其脂质体的主要成分。本文的在线版本（10.1186/s12870-017-1103-6）包含补充材料，可供授权用户使用。
Avocado fruit contains aliphatic acetogenins (oft-acetylated, odd-chain fatty alcohols) with promising bioactivities for both medical and food industries. However, we have scarce knowledge about their metabolism. The present work aimed to study changes in acetogenin profiles from mesocarp, lipid-containing idioblasts, and seeds from ‘Hass’ cultivar during fruit development, germination, and three harvesting years. An untargeted LC-MS based lipidomic analysis was also conducted to profile the lipidome of avocado fruit in each tissue. The targeted analysis showed that acetogenin profiles and contents remained unchanged in avocado mesocarp during maturation and postharvest ripening, germination, and different harvesting years. However, a shift in the acetogenin profile distribution, accompanied with a sharp increase in concentration, was observed in seed during early maturation. Untargeted lipidomics showed that this shift was accompanied with remodeling of glycerolipids: TAGs and DAGs decreased during fruit growing in seed. Remarkably, the majority of the lipidome in mature seed was composed by acetogenins; we suggest that this tissue is able to synthesize them independently from mesocarp. On the other hand, lipid-containing idioblasts accumulated almost the entire acetogenin pool measured in the whole mesocarp, while only having 4% of the total fatty acids. The lipidome of this cell type changed the most when the fruit was ripening after harvesting, TAGs decreased while odd-chain DAGs increased. Notably, idioblast lipidome was more diverse than that from mesocarp. Evidence shown here suggests that idioblasts are the main site of acetogenin biosynthesis in avocado mesocarp. This work unveiled the prevalence of aliphatic acetogenins in the avocado fruit lipidome and evidenced TAGs as initial donors of the acetogenin backbones in its biosynthesis. It also sets evidence for acetogenins being included in future works aimed at characterizing the avocado seed, as they are a main component of their lipidome. The online version of this article (10.1186/s12870-017-1103-6) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
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影响因子：
3.9
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