Monitoring the chemical production of citrus-derived bioactive 5-demethylnobiletin using surface-enhanced Raman spectroscopy.
Monitoring the chemical production of citrus-derived bioactive 5-demethylnobiletin using surface-enhanced Raman spectroscopy.
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DOI：
10.1021/jf4027475
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发表时间：
2013-08-28
影响因子：
6.1
通讯作者：
He L
中科院分区：
文献类型：
--
作者：
Zheng J;Fang X;Cao Y;Xiao H;He L
为了开发一种准确、便捷的方法来监测柑橘来源的生物活性 5-去甲基川陈皮素的去甲基化反应的生产，我们将表面增强拉曼光谱 (SERS) 方法与传统的 HPLC 方法进行了比较。我们的结果表明，基于基质和基于溶液的 SERS 方法与 HPLC 方法的相关性非常好。与基质方法相比，溶液方法产生了更低的校准均方根误差和更高的相关系数。该溶液法利用类似“亲和色谱”的程序，根据反应物川陈皮素与产物 5-去甲基胆汁素与银枝晶的不同结合亲和力将其分离。发现基质方法更简单、更快速地收集样品的 SERS“指纹”光谱，因为不需要样品和银之间的孵育，并且只需要痕量的样品。我们的结果表明，SERS 方法在方便、快速地表征和定量 5-去甲基川陈皮素生产方面优于 HPLC 方法。
To develop an accurate and convenient method for monitoring the production of citrus-derived bioactive 5-demethylnobiletin from demethylation reaction of nobiletin, we compared surface enhanced Raman spectroscopy (SERS) methods with a conventional HPLC method. Our results show that both the substrate-based and solution-based SERS methods correlated with HPLC method very well. The solution method produced lower root mean square error of calibration and higher correlation coefficient than the substrate method. The solution method utilized an ‘affinity chromatography’-like procedure to separate the reactant nobiletin from the product 5-demthylnobiletin based on their different binding affinity to the silver dendrites. The substrate method was found simpler and faster to collect the SERS ‘fingerprint’ spectra of the samples as no incubation between samples and silver was needed and only trace amount of samples were required. Our results demonstrated that the SERS methods were superior to HPLC method in conveniently and rapidly characterizing and quantifying 5-demethylnobiletin production.
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影响因子：
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