Expression of ZmHDZ4, a Maize Homeodomain-Leucine Zipper I Gene, Confers Tolerance to Drought Stress in Transgenic Rice
Expression of ZmHDZ4, a Maize Homeodomain-Leucine Zipper I Gene, Confers Tolerance to Drought Stress in Transgenic Rice
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玉米同源结构域亮氨酸拉链 I 基因 ZmHDZ4 的表达赋予转基因水稻对干旱胁迫的耐受性
DOI：
10.1007/s11105-015-0970-y
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发表时间：
2016-01
影响因子：
2.1
通讯作者：
Cheng Beijiu
中科院分区：
文献类型：
--
作者：
Wu Ji;ong;Zhou Wei;Gong Xuefeng;Cheng Beijiu
由于全球气温上升和降雨不可预测，气候变化预计将成为农作物产量的主要威胁。因此，鉴定一些能够帮助植物应对干旱胁迫的新基因是很重要的。本研究从玉米中分离到干旱诱导的同源结构域亮氨酸拉链（HD-Zip） 1基因ZmHDZ4，并对其在干旱胁迫中的作用进行了鉴定。瞬时表达实验表明，ZmHDZ4定位于细胞核。酵母单杂交实验表明，ZmHDZ4具有反式激活活性，最小激活结构域为ZmHDZ4-1。我们发现ZmHDZ4在水稻中过表达可以增强水稻对干旱的耐受性和对ABA的敏感性。与野生型植株相比，表达zmhdz4的转基因植株在干旱胁迫条件下具有较低的相对电解质泄漏（REL）、较低的丙二醛（MDA）水平和较高的脯氨酸含量，这可能是其抗旱性增强的原因之一。综上所述，这些结果表明ZmHDZ4作为一个转录调控因子可以积极影响植物的抗旱性。因此，ZmHDZ4是一个极好的候选基因，在提高作物抗旱性的分子育种中具有潜在的应用前景。
Climate change is predicted to be a major threat to crop yield due to increasing global temperatures and periods of unpredictable rainfall. Therefore, it is important to identify some new genes that can help plants cope with drought stress. In this study, a drought-induced homeodomain-leucine zipper (HD-Zip) I gene, ZmHDZ4, was isolated from maize and characterized for its role in drought stress. Transient expression experiments showed that ZmHDZ4 is localized to the nucleus. Yeast one-hybrid assays demonstrated that ZmHDZ4 has trans-activation activity, and that the minimal activation domain was ZmHDZ4-1. We found that overexpression of ZmHDZ4 in rice can enhance tolerance to drought and increase sensitivity to abscisic acid (ABA). Compared to wild-type plants, ZmHDZ4-expressing transgenic plants had lower relative electrolyte leakage (REL), lower malondialdehyde (MDA) levels, and increased proline contents under drought stress conditions, all of which may contribute to enhanced drought tolerance. Taken together, these results suggest that ZmHDZ4 functions as a transcriptional regulator that can positively affect plant drought tolerance. Thus, ZmHDZ4 is an excellent candidate gene with potential applications in molecular breeding to improve crop drought tolerance.
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影响因子：
7.2
作者：
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通讯作者：
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影响因子：
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影响因子：
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DOI：
10.1006/bbrc.2001.4904
发表时间：
2001-06-01
影响因子：
3.1
作者：
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通讯作者：
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