Overexpression of a novel MADS-box gene SlFYFL delays senescence, fruit ripening and abscission in tomato.
Overexpression of a novel MADS-box gene SlFYFL delays senescence, fruit ripening and abscission in tomato.
复制标题
新型 MADS-box 基因 SlFYFL 的过度表达可延缓番茄衰老、果实成熟和脱落
DOI：
10.1038/srep04367
复制
发表时间：
2014-03-13
影响因子：
4.6
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Xie Q;Hu Z;Zhu Z;Dong T;Zhao Z;Cui B;Chen G
MADS结构域蛋白是重要的转录因子，参与植物的许多生物学过程。本研究克隆了番茄MADS-box基因SlFYFL。SlFYFL在番茄的所有组织中均有表达，在成熟叶、不同时期的果实、AZ（分离区）和萼片中表达量显著高于其它组织。35 S：FYFL番茄叶片衰老和果实成熟延迟，耐贮性提高，萼片变长。35 S：FYFL果实类胡萝卜素积累减少，乙烯含量、乙烯生物合成和响应基因表达下调。35 S：FYFL植株的离区不能正常形成，离区发育相关基因表达下降。酵母双杂交实验表明，SlFYFL蛋白可分别与SlMADS-RIN、SlMADS 1和SlJOINTLESS相互作用。这些结果表明，SlFYFL的过表达调控果实成熟和AZ的发展，通过与成熟和成熟区相关的MADS盒蛋白的相互作用。
MADS-domain proteins are important transcription factors involved in many biological processes of plants. In our study, a tomato MADS-box gene, SlFYFL, was isolated. SlFYFL is expressed in all tissues of tomato and significantly higher in mature leave, fruit of different stages, AZ (abscission zone) and sepal. Delayed leaf senescence and fruit ripening, increased storability and longer sepals were observed in 35S:FYFL tomato. The accumulation of carotenoid was reduced, and ethylene content, ethylene biosynthetic and responsive genes were down-regulated in 35S:FYFL fruits. Abscission zone (AZ) did not form normally and abscission zone development related genes were declined in AZs of 35S:FYFL plants. Yeast two-hybrid assay revealed that SlFYFL protein could interact with SlMADS-RIN, SlMADS1 and SlJOINTLESS, respectively. These results suggest that overexpression of SlFYFL regulate fruit ripening and development of AZ via interactions with the ripening and abscission zone-related MADS box proteins.
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影响因子：
3.9
作者：
Fraser, Paul D.;Enfissi, Eugenia M. A.;Bramley, Peter M.
通讯作者：
Bramley, Peter M.
影响因子：
6.9
作者：
Forth, Daniel;Pyke, Kevin A.
通讯作者：
Pyke, Kevin A.
DOI：
10.1073/pnas.76.1.170
发表时间：
1979-01-01
影响因子：
11.1
作者：
ADAMS, DO;YANG, SF
通讯作者：
YANG, SF
影响因子：
7.2
作者：
GRBIC, V;BLEECKER, AB
通讯作者：
BLEECKER, AB
影响因子：
7.2
作者：
Chung, Mi-Young;Vrebalov, Julia;Giovannoni, James
通讯作者：
Giovannoni, James