Bacteroides ovatus colonization influences the abundance of intestinal short chain fatty acids and neurotransmitters.
Bacteroides ovatus colonization influences the abundance of intestinal short chain fatty acids and neurotransmitters.
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DOI：
10.1016/j.isci.2022.104158
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发表时间：
2022-05-20
期刊：
影响因子：
5.8
通讯作者：
Engevik, Melinda A.
中科院分区：
文献类型：
--
作者：
Horvath, Thomas D.;Ihekweazu, Faith D.;Haidacher, Sigmund J.;Ruan, Wenly;Engevik, Kristen A.;Fultz, Robert;Hoch, Kathleen M.;Luna, Ruth Ann;Oezguen, Numan;Spinler, Jennifer K.;Haag, Anthony M.;Versalovic, James;Engevik, Melinda A.
肠道微生物可以合成多种神经活性代谢物。通过LC-MS/MS分析了肠道寄生卵形拟杆菌在体外和体内产生的神经活性化合物。我们发现，B. ovatus产生乙酸、丙酸、异丁酸和异戊酸。在体外，卵形B. ovatus消耗色氨酸和谷氨酸，并合成神经活性化合物谷氨酰胺和GABA。与我们基于LC-MS/MS的体外数据一致，我们观察到与无菌对照组相比，卵圆杆菌单相关小鼠肠道中乙酸、丙酸、异丁酸和异戊酸水平升高。B. ovatus mono-associated mice.与无菌对照组相比，B. ovatus单联合也增加了肠道GABA的浓度，降低了色氨酸和谷氨酰胺的浓度。计算网络分析揭示了SCFAs，神经活性化合物和殖民地位之间的独特联系。这些结果突出了微生物定植和肠道神经递质浓度之间的联系，表明B. ovatus选择性地影响肠道神经递质的存在。卵形拟杆菌在知源动物体内调节关键神经活性化合物神经科学;微生物学;微生物组
Gut microbes can synthesize multiple neuro-active metabolites. We profiled neuro-active compounds produced by the gut commensal Bacteroides ovatus in vitro and in vivo by LC-MS/MS. We found that B. ovatus generates acetic acid, propionic acid, isobutyric acid, and isovaleric acid. In vitro, B. ovatus consumed tryptophan and glutamate and synthesized the neuro-active compounds glutamine and GABA. Consistent with our LC-MS/MS-based in vitro data, we observed elevated levels of acetic acid, propionic acid, isobutyric acid, and isovaleric acid in the intestines of B. ovatus mono-associated mice compared with germ-free controls. B. ovatus mono-association also increased the concentrations of intestinal GABA and decreased the concentrations of tryptophan and glutamine compared with germ-free controls. Computational network analysis revealed unique links between SCFAs, neuro-active compounds, and colonization status. These results highlight connections between microbial colonization and intestinal neurotransmitter concentrations, suggesting that B. ovatus selectively influences the presence of intestinal neurotransmitters. Bacteroides ovatus modulates key neuro-active compounds in vivo in gnotobiotic animals Neuroscience; Microbiology; Microbiome
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发表时间：
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影响因子：
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作者：
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