Genomic and Morphological Differentiation of Spirit Producing Agave angustifolia Traditional Landraces Cultivated in Jalisco, Mexico.

Genomic and Morphological Differentiation of Spirit Producing Agave angustifolia Traditional Landraces Cultivated in Jalisco, Mexico.
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DOI:
10.3390/plants11172274
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发表时间:
2022-08-31
期刊:
Plants (Basel, Switzerland)
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其他
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传统的龙舌兰酒,如梅斯卡尔或龙舌兰酒,在墨西哥各地都是用不同种类的龙舌兰酒生产的。其中,A.鉴于其农业延伸,狭叶是最受欢迎的。A.狭叶龙舌兰(angustifolia)是一种广泛分布于北美至中美洲的野生植物,与龙舌兰(tequila agave A.)龙舌兰酒在墨西哥的不同地区,A.沙枣在不同类型和管理水平下栽培,尽管传统生产者识别出几种地方品种,但对于未经训练的眼睛来说,没有可察觉的差异。在采访了来自墨西哥哈利斯科不同地区的生产商后,我们对A.将angustifolia植物划分为不同的地方品种,测量了几个形态性状,并在全基因组水平上表征了它们的遗传分化和多样性。我们纳入了标识为A的其他样本。tequilana和A. rhodacantha来评估它们与A.狭叶与以前的研究相比,我们的约20K高质量的非连锁SNP库提供了更多的信息,并帮助我们区分与民族植物学地方品种一致的不同遗传组。我们没有发现证据从遗传上界定A。tequilana、龙舌兰A. rhodacantha和A.狭叶我们的大型基因组水平数据集允许更好地了解重要的A.狭叶传统和本地地方品种。
Traditional agave spirits such as mezcal or tequila are produced all over Mexico using different species of Agave. Amongst them, A. angustifolia is the most popular given its agricultural extension. A. angustifolia is a wild species extensively distributed from North to Central America, and previous studies show that it is highly related to the tequila agave A. tequilana. In different regions of Mexico, A. angustifolia is cultivated under different types and levels of management, and although traditional producers identify several landraces, for the non-trained eye there are no perceivable differences. After interviews with producers from different localities in Jalisco, Mexico, we sampled A. angustifolia plants classified as different landraces, measured several morphological traits, and characterized their genetic differentiation and diversity at the genome-wide level. We included additional samples identified as A. tequilana and A. rhodacantha to evaluate their relationship with A. angustifolia. In contrast with previous studies, our pool of ca 20K high quality unlinked SNPs provided more information and helped us to distinguish different genetic groups that are congruent with the ethnobotanical landraces. We found no evidence to genetically delimitate A. tequilana, A. rhodacantha and A. angustifolia. Our large genome level dataset allows a better understanding of the genetic identity of important A. angustifolia traditional and autochthonous landraces.
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