A Carbon Fixation Enhanced Chlamydomonas reinhardtii Strain for Achieving the Double-Win Between Growth and Biofuel Production Under Non-stressed Conditions.
A Carbon Fixation Enhanced Chlamydomonas reinhardtii Strain for Achieving the Double-Win Between Growth and Biofuel Production Under Non-stressed Conditions.
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一种增强碳固定的莱茵衣藻菌株，可在非胁迫条件下实现生长和生物燃料生产的双赢
DOI：
10.3389/fbioe.2020.603513
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发表时间：
2020
影响因子：
5.7
通讯作者：
Tian J
中科院分区：
文献类型：
--
作者：
Zhu Z;Cao H;Li X;Rong J;Cao X;Tian J
胁迫培养被广泛应用于微藻生物燃料研发中，但在一定程度上抑制了微藻的生长，限制了微藻的整体生产力。生长与蓄能化合物积累之间的平衡是菌种选择或构建与培养优化相结合的目标。本研究将一株叶绿体型甘油醛-3-磷酸脱氢酶（cGAPDH）过表达的莱茵衣藻（Chlamydomonas reinhardtii）工程菌株命名为P3-GAPDH，在海藻站平台上培养。与野生型C. reinhardtii CC137c相比，在Tris-acetate-phosphate （TAP）培养基中，96 h内WT和P3-GAPDH的最高密度分别为1.23±0.13和1.74±0.09 g L-1，最大生物量生产力分别为24.30±1.65和28.54±1.43 mg L-1 h - 1。在能量储存化合物方面，P3-GAPDH碳水化合物和脂肪酸含量均增加了一倍，碳水化合物含量从0.13±0.02 g L-1增加到0.26±0.04 g L-1，脂肪酸含量从0.08±0.01 g L-1增加到0.16±0.01 g L-1，其中多不饱和脂肪酸含量增加了65.8%。通过对叶绿素荧光动力学参数Fv/Fm、Fv ' /Fm和培养物pH的持续监测，发现过表达cGAPDH促进了卡尔文循环，促进了碳转化和随后的储能化合物积累。C. reinhardtii P3-GAPDH菌株表现出作为高碳转化能力的优良基质的潜力。
The stressed cultivations are widely used in microalgae R&D for the biofuel production with the repress on growth to a certain degree, which limits the overall productivity. The balance between the growth and energy storage compounds accumulation is a target needing the combination of both strain selection or construction and culture optimization. Here, an engineered strain of Chlamydomonas reinhardtii, in which the chloroplast type glyceraldehyde-3-phosphate dehydrogenase (cGAPDH) was overexpressed and named as P3-GAPDH, was cultured on the Algal Station platform. Compared with wild type (WT), C. reinhardtii CC137c, in Tris-acetate-phosphate (TAP) medium, the highest density of WT and P3-GAPDH were 1.23 ± 0.13 and 1.74 ± 0.09 g L–1 within 96 h, and the maximum biomass productivity was 24.30 ± 1.65 and 28.54 ± 1.43 mg L–1 h–1, respectively. In terms of the energy storage compounds, both carbohydrate and fatty acids content doubled in P3-GAPDH, from 0.13 ± 0.02 to 0.26 ± 0.04 g L–1 for carbohydrate and from 0.08 ± 0.01 to 0.16 ± 0.01 g L–1 for fatty acids, among which poly unsaturated fatty acids increased by 65.8%. Together with the continuous monitor of the chlorophyll fluorescence dynamics parameters Fv/Fm and Fv’/Fm’ and pH of culture, enhanced Calvin cycle by overexpressed cGAPDH promoted the carbon conversion and subsequent energy storage compounds accumulation. C. reinhardtii P3-GAPDH strain showed the potential as a good chassis with high carbon conversion ability.
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影响因子：
6.3
作者：
Kim, Eun Jae;Kim, Sanghee;Han, Se Jong
通讯作者：
Han, Se Jong
影响因子：
4.1
作者：
Scoma, Alberto;Giannelli, Luca;Torzillo, Giuseppe
通讯作者：
Torzillo, Giuseppe
影响因子：
7.2
作者：
Kim, MS;Baek, JS;Kim, SC
通讯作者：
Kim, SC
影响因子：
5.6
作者：
Yang M;Fan Y;Wu PC;Chu YD;Shen PL;Xue S;Chi ZY
通讯作者：
Chi ZY
DOI：
10.1111/j.1432-1033.2004.04437.x
发表时间：
2004-12-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Graciet, E;Mulliert, G;Gontero, B
通讯作者：
Gontero, B