Poly(oligo(ethylene glycol) methyl ether methacrylate) Capped pH-Responsive Poly(2-(diethylamino)ethyl methacrylate) Brushes Grafted on Mesoporous Silica Nanoparticles as Nanocarrier.
Poly(oligo(ethylene glycol) methyl ether methacrylate) Capped pH-Responsive Poly(2-(diethylamino)ethyl methacrylate) Brushes Grafted on Mesoporous Silica Nanoparticles as Nanocarrier.
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DOI：
10.3390/polym13050823
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发表时间：
2021-03-08
期刊：
影响因子：
5
通讯作者：
Alswieleh AM
中科院分区：
文献类型：
--
作者：
Alotaibi KM;Almethen AA;Beagan AM;Alfhaid LH;Ahamed M;El-Toni AM;Alswieleh AM
本文基于介孔二氧化硅纳米粒子（MSNs）开发了一种新的pH响应性纳米系统用于癌症治疗。聚（2-（二乙基氨基）乙基甲基丙烯酸酯）（PDEAEMA）接枝在它们的外表面上，并作为看门人，随后通过半胱氨酸（MSN-PDEAEMA-Cys）和聚（低聚（乙二醇）甲基醚甲基丙烯酸酯）（MSN-PDEAEMA-Cys-POEGMEMA）的聚合物的后续改性。这些纳米载体的物理化学性质，其特征在于使用扫描和透射电子显微镜（SEM和TEM），傅里叶变换红外光谱（FTIR），X射线光电子能谱（XPS），和动态光散射（DLS）。合成的纳米粒子分散性好，粒径约为100 nm。200纳米。所获得的XPS结果证实了通过分别在286.5和288.5 eV处增加C-O和C=O基团的峰强度，用Cys和POEGMEMA成功地修饰了MSN-PDEAEMA。抗癌药物阿霉素（DOX）被封装到所制造的纳米平台中。在生理pH值为7.4时，DOX的释放量有限（10%），而累积药物释放约为10%。在酸性介质中30 h后达到35%。MTT细胞系用于评估未加载和加载DOX的制造的纳米平台的细胞毒性。在这些纳米材料上负载DOX后，它们对人类肝癌细胞显示出显著的毒性。这些结果表明，所制备的纳米结构材料也表现出良好的生物相容性，它们可以作为抗癌药物的纳米载体。
In this paper, a new pH-responsive nanosystem based on mesoporous silica nanoparticles (MSNs) was developed for cancer therapy. Poly(2-(diethylamino) ethyl methacrylate) (PDEAEMA) was grafted on their outer surface and acts as a gatekeeper, followed by subsequent modification of the polymer by cysteine (MSN-PDEAEMA-Cys) and poly(oligo(ethylene glycol) methyl ether methacrylate) (MSN-PDEAEMA-Cys-POEGMEMA). The physicochemical properties of these nanocarriers were characterized using scanning and transmission electron microscopies (SEM and TEM), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), and dynamic light scattering (DLS). The synthesized nanoparticles were well-dispersed with a diameter of ca. 200 nm. The obtained XPS results confirm the successful modification of MSN-PDEAEMA with Cys and POEGMEMA by increasing the peak intensity of C–O and C=O groups at 286.5 and 288.5 eV, respectively. An anti-cancer drug, doxorubicin (DOX), was encapsulated into the fabricated nanoplatform. The DOX release amount at physiological pH of 7.4 was limited (10%), while an accumulation drug release of ca. 35% was accomplished after 30 h in acidic media. The MTT cell line was used to assess the cytotoxicity of the unloaded and DOX-loaded fabricated nanoplatforms. Upon loading of DOX on these nanomaterials, they showed significant toxicity to human liver cancer cells. These results suggest that the prepared nano-structured materials showed good biocompatibility as well, and they can serve as nanocarriers for the delivery of anti-cancer drugs.
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