Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar.
Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar.
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DOI：
10.1038/s41467-023-39930-3
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发表时间：
2023-07-18
影响因子：
16.6
通讯作者：
Lin J
中科院分区：
文献类型：
--
作者：
Guo Y;Wang S;Yu K;Wang HL;Xu H;Song C;Zhao Y;Wen J;Fu C;Li Y;Wang S;Zhang X;Zhang Y;Cao Y;Shao F;Wang X;Deng X;Chen T;Zhao Q;Li L;Wang G;Grünhofer P;Schreiber L;Li Y;Song G;Dixon RA;Lin J
木质纤维素原料转化为可发酵糖用于生物燃料生产是低效的，并且大多数提高效率的策略直接针对木质素生物合成，具有相关的负面生长影响。在这里，我们证明了，对于实验室和田间生长的植物，Pag-miR 408在杨树（白杨×腺白杨）中的表达显著增强了糖化作用，不需要酸预处理，同时促进植物生长。过表达的植物表现出细胞壁对纤维素酶和支架蛋白纤维素结合模块的可接近性增加。相反，Pag-miR 408功能丧失白杨显示降低的细胞壁可及性。Pag-miR 408的过表达靶向三种Pag-LACCASES，延迟木质化，并适度降低木质素含量、S/G比和木质素聚合度。同时，漆酶功能丧失突变体在木质部中表现出显著增加的生长和细胞壁可及性。我们的研究显示了Pag-miR 408如何调节木质化和次生生长，并提出了一种有效的方法来提高主要生物能源作物的生物质产量和糖化效率。修饰植物木质素途径以提高糖化效率通常与生长惩罚相关。在这里，作者表明，Pag-miR 408在白杨中的过表达导致实验室和田间条件下糖化效率和生长的增强，并且漆酶基因是Pag-miR 408的靶标。
The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop. Modifying plant lignin pathway to enhance saccharification efficiency is often associated with growth penalty. Here, the authors show that overexpression of Pag-miR408 in poplar leads to enhanced saccharification efficiency and growth in both laboratory and field conditions, and laccase genes are the targets of Pag-miR408.
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影响因子：
11.4
作者：
Pan, Jiawei;Huang, Dahui;Li, Lei
通讯作者：
Li, Lei
影响因子：
3.6
作者：
Jackson, Lisa A.;Shadle, Gail L.;Dixon, Richard A.
通讯作者：
Dixon, Richard A.
DOI：
10.1073/pnas.2010911118
发表时间：
2021-02-02
影响因子：
11.1
作者：
Liu C;Yu H;Rao X;Li L;Dixon RA
通讯作者：
Dixon RA
影响因子：
3.1
作者：
BLAKENEY, AB;HARRIS, PJ;STONE, BA
通讯作者：
STONE, BA
影响因子：
2.7
作者：
Ding, Shi-You;Xu, Qi;Himmel, Michael E.
通讯作者：
Himmel, Michael E.