Safety and serological response to a matrix gene-deleted rabies virus-based vaccine vector in dogs.
Safety and serological response to a matrix gene-deleted rabies virus-based vaccine vector in dogs.
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DOI：
10.1016/j.vaccine.2014.01.043
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发表时间：
2014-03-26
期刊：
影响因子：
5.5
通讯作者：
Schnell, Matthias J.
中科院分区：
文献类型：
--
作者：
McGettigan, James P.;David, Frederic;Figueiredo, Monica Dias;Minke, Jules;Mebatsion, Teshome;Schnell, Matthias J.
在全球范围内，狂犬病病毒(RABV)导致的人类接触和死亡中，狗占了大部分。在这份报告中，我们证明了一种基于复制缺陷的基质基因缺失的RABV疫苗(RABV-M)是安全的，并且在狗的单次接种后诱导快速而有效的VNA滴度。用106或107个病灶形成单位(FFU)的RABV-M单剂免疫14天后，平均VNA滴度分别达到3.02或5.11国际单位(IU/ml)，到免疫后第70天，两种疫苗的VNA滴度均为0.5IU/ml，达到满意免疫的水平。重要的是，在任何接种了RABV-M疫苗的狗身上都没有观察到系统或局部反应。通过大规模接种狗狂犬病受到以下因素的阻碍：资源有限，需要经常重复接种疫苗，而且在世界一些地区，疫苗质量较差。我们在狗身上的初步安全性和免疫原性数据表明，RABV-M可能会在疫苗接种活动中补充目前使用的基于RABV的灭活疫苗，帮助在接种狗中获得100%的应答，从而增加整体疫苗接种覆盖率。
Dogs account for the majority of human exposures and deaths due to rabies virus (RABV) worldwide. In this report, we show that a replication-deficient RABV-based vaccine in which the matrix gene is deleted (RABV- M) is safe and induces rapid and potent VNA titers after a single inoculation in dogs. Average VNA titers peaked at 3.02 or 5.11 International Units (IU/ml) by 14 days post-immunization with a single dose of 106 or 107 focus forming units (ffu), respectively, of RABV- M. By day 70 post immunization, all dogs immunized with either dose of vaccine showed VNA titers >0.5 IU/ml, the level indicative of a satisfactory immunization. Importantly, no systemic or local reactions were noted in any dog immunized with RABV- M. The elimination of dog rabies through mass vaccination is hindered by limited resources, requirement for repeat vaccinations often for the life of a dog, and in some parts of the world, inferior vaccine quality. Our preliminary safety and immunogenicity data in dogs suggest that RABV- M might complement currently used inactivated RABV-based vaccines in vaccination campaigns by helping to obtain 100% response in vaccinated dogs, thereby increasing overall vaccination coverage.
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