A genome-wide resource of cell cycle and cell shape genes of fission yeast.

A genome-wide resource of cell cycle and cell shape genes of fission yeast.
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DOI:
10.1098/rsob.130053
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发表时间:
2013-05-22
期刊:
影响因子:
5.8
通讯作者:
Nurse P
Nurse P
中科院分区:
生物学2区
文献类型:
--
作者:
Hayles J;Wood V;Jeffery L;Hoe KL;Kim DU;Park HO;Salas-Pino S;Heichinger C;Nurse P

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为了确定细胞周期和细胞形状所需的基因的接近完整的集合,我们已经视觉上筛选了4843个裂殖酵母缺失突变体(总蛋白质编码基因的95.7%)的全基因组基因缺失文库,以了解它们对这些过程的影响。总共有513个基因已被确定为细胞周期进程所需的,其中276个以前没有被描述为细胞周期基因。另外333个基因的缺失导致细胞形状的特定改变,另外524个基因导致通常畸形的细胞。在这里,我们提供了基因缺失的第一个真核生物资源,它描述了细胞周期和细胞形状所需的一组接近全基因组的基因。
To identify near complete sets of genes required for the cell cycle and cell shape, we have visually screened a genome-wide gene deletion library of 4843 fission yeast deletion mutants (95.7% of total protein encoding genes) for their effects on these processes. A total of 513 genes have been identified as being required for cell cycle progression, 276 of which have not been previously described as cell cycle genes. Deletions of a further 333 genes lead to specific alterations in cell shape and another 524 genes result in generally misshapen cells. Here, we provide the first eukaryotic resource of gene deletions, which describes a near genome-wide set of genes required for the cell cycle and cell shape.
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