Quantitative proteomics reveals that distant recurrence-associated protein R-Ras and Transgelin predict post-surgical survival in patients with Stage III colorectal cancer.
Quantitative proteomics reveals that distant recurrence-associated protein R-Ras and Transgelin predict post-surgical survival in patients with Stage III colorectal cancer.
复制标题
定量蛋白质组学揭示远处复发相关蛋白 R-Ras 和 Transgelin 可预测 III 期结直肠癌患者的术后生存率
DOI：
10.18632/oncotarget.9701
复制
发表时间：
2016-07-12
期刊：
影响因子：
--
通讯作者：
Ge W
中科院分区：
文献类型：
--
作者：
Xu L;Gao Y;Chen Y;Xiao Y;He Q;Qiu H;Ge W
手术切除辅以辅助化疗是目前III期结直肠癌（CRC）的首选治疗方法。然而，多达48%的接受根治性切除的患者最终患有无法治愈的远处复发。为了研究III期结直肠癌术后远端复发的分子机制，我们使用基于TMT的定量质谱法鉴定了总共146个与III期结直肠癌远端复发相关的差异表达蛋白（DEPs）。在这些DEP中，然后使用免疫组织化学（IHC）在192个个体标本中验证R-Ras和Transgelin的表达改变。Kaplan-Meier分析显示R-Ras和Transgelin水平与5年总生存期（OS）和无病生存期（DFS）显著相关，多因素Cox回归分析显示R-Ras和Transgelin分别是影响OS和DFS的独立预后因素。总之，本研究确定了参与远端复发的潜在生化参与者，并表明R-Ras和Transgelin是III期CRC的潜在术后预后生物标志物。该蛋白质组学数据已提交给Proteome Xchange，登录号为PXD 002903。
Surgical resection supplemented with adjuvant chemotherapy is the current preferred treatment for Stage III colorectal cancer (CRC). However, as many as 48% of patients who undergo curative resection eventually suffer from incurable distant recurrence. To investigate the molecular mechanisms involved in Stage III CRC post-surgical distant recurrence, we identified a total of 146 differentially expressed proteins (DEPs) associated with distant recurrence in Stage III CRC using TMT-based quantitative mass spectrometry. Among these DEPs, the altered expressions of R-Ras and Transgelin were then validated in 192 individual specimens using immunohistochemistry (IHC). Furthermore, Kaplan-Meier analysis revealed that the levels of R-Ras and Transgelin were significantly associated with 5-year overall survival (OS) and disease-free survival (DFS), and multivariate Cox-regression analyses revealed that R-Ras and Transgelin were independent prognostic factors for OS and DFS, respectively. In conclusion, this study identified potential biochemical players involved in distant recurrence and indicates that R-Ras and Transgelin are potential post-surgical prognostic biomarkers for Stage III CRC. This proteomics data have been submitted to Proteome Xchange under accession number PXD002903.
登录
查看更多内容
DOI：
10.1016/j.biocel.2008.02.011
发表时间：
2009-03-01
影响因子：
4
作者：
Assinder, Stephen J.;Stanton, Jo-Ann L.;Prasad, Priya D.
通讯作者：
Prasad, Priya D.
Ras 相关蛋白 Rab 5C 依赖性内吞作用和糖酵解作用在顺铂耐药卵巢癌细胞系中的下调
影响因子：
7
作者：
Jin, Lixu;Huo, Yi;Deng, Haiteng
通讯作者：
Deng, Haiteng
影响因子：
254.7
作者：
Torre, Lindsey A.;Bray, Freddie;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin
影响因子：
4.8
作者：
Lin, Ying;Buckhaults, Phillip J.;Dynan, William S.
通讯作者：
Dynan, William S.
癌症的分子起源：结直肠癌的分子基础。
DOI：
10.1056/nejmra0804588
发表时间：
2009-12-17
期刊：
The New England journal of medicine
影响因子：
--
作者：
Markowitz SD;Bertagnolli MM
通讯作者：
Bertagnolli MM