Ambient mass spectrometry in metabolomics.
Ambient mass spectrometry in metabolomics.
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DOI：
10.1039/c7an00700k
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发表时间：
2017-08-21
期刊：
影响因子：
--
通讯作者：
Fernández FM
中科院分区：
文献类型：
--
作者：
Clendinen CS;Monge ME;Fernández FM
自解吸电喷雾电离（DESI）质谱（MS）引入以来，环境MS方法在从健康到食品科学的各种领域中的应用越来越多。环境质谱在代谢组学中越来越受欢迎，它提供了有限的样品制备，液体，固体和气体的快速和直接分析，原位和体内分析以及成像。代谢组由不断变化的小分子（<1.5KDa）组成。这些包括作为初级代谢途径一部分的内源性分子、具有特定功能（如信号传导）的次级代谢物、纳入饮食中或由环境暴露产生的化学物质以及与微生物组相关的代谢物。表征该分子组群的响应变化是任何代谢组学研究的主要目标。通过调整实验参数，可以选择性地解吸和电离一系列化学和物理性质的代谢物，并随后以更高的速度和灵敏度进行分析。本文综述了各种环境质谱技术在代谢组学常用的四个主要领域的广泛应用。环境质谱法作为一种高通量的替代传统联用方法，在不断发展的代谢组学领域发挥着越来越重要的作用。
Since the introduction of desorption electrospray ionization (DESI) mass spectrometry (MS), ambient MS methods have seen increased use in a variety of fields from health to food science. Increasing its popularity in metabolomics, ambient MS offers limited sample preparation, rapid and direct analysis of liquids, solids, and gases, in situ and in vivo analysis, and imaging. The metabolome consists of a constantly changing collection of small (<1.5KDa) molecules. These include endogenous molecules that are part of primary metabolism pathways, secondary metabolites with specific functions such as signaling, chemicals incorporated in the diet or resulting from environmental exposures, and metabolites associated with the microbiome. Characterization of the responsive changes of this molecule cohort is the principle goal in any metabolomics study. With adjustments to experimental parameters, metabolites across a range of chemical and physical properties can be selectively desorbed and ionized and subsequently analyzed with increased speed and sensitivity. This review covers the broad applications of a variety of ambient MS techniques in four primary fields in which metabolomics is commonly employed. Ambient mass spectrometry continues to grow as a high-throughput alternative to more traditional hyphenated methods, playing an increasingly-relevant role in the growing field of metabolomics.
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影响因子：
6.1
作者：
Cajka, Tomas;Danhelova, Hana;Hajslova, Jana
通讯作者：
Hajslova, Jana
影响因子：
7.4
作者：
Cody, RB;Laramée, JA;Durst, HD
通讯作者：
Durst, HD
实时质谱直接分析结合单滴液-液-液微萃取快速分析果汁中的多种植物激素
影响因子：
4.3
作者：
Bai, Yu;Zhang, Jialing;Liu, Huwei
通讯作者：
Liu, Huwei
影响因子：
6
作者：
Cody, Robert B.;Dane, A. John;Nkansah, Kofi
通讯作者：
Nkansah, Kofi
DOI：
10.1073/pnas.93.14.7003
发表时间：
1996-07-09
影响因子：
11.1
作者：
Berkenkamp, S;Karas, M;Hillenkamp, F
通讯作者：
Hillenkamp, F