Molecular sensing with magnetic nanoparticles using magnetic spectroscopy of nanoparticle Brownian motion.
Molecular sensing with magnetic nanoparticles using magnetic spectroscopy of nanoparticle Brownian motion.
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DOI：
10.1016/j.bios.2013.06.049
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发表时间：
2013-12-15
影响因子：
12.6
通讯作者：
Weaver, John B.
中科院分区：
文献类型：
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作者：
Zhang, Xiaojuan;Reeves, Daniel B.;Perreard, Irina M.;Kett, Warren C.;Griswold, Karl E.;Gimi, Barjor;Weaver, John B.
功能化的磁性纳米颗粒（mNPs）在生物传感和其他生物医学应用中显示出了希望。在这里，我们使用功能化的mNPs开发一个高度敏感的，通用的传感策略所需的实际生物测定和潜在的体内分析。我们提出了一种新的传感方案的基础上的纳米粒子布朗运动（MSB）的磁谱定量检测分子目标。MSB使用振荡mNP的谐波作为旋转运动自由度的度量，从而反映mNP的束缚态。谐波可以在5秒内从纳克量的铁中在体内检测到。使用链霉亲和素-生物素结合系统，我们表明，目前的MSB技术的检测限低于150 pM（0.075皮摩尔），这是更敏感的比以前报道的技术的基础上mNP检测。使用与两个抗凝血酶DNA适体缀合的mNP，我们表明凝血酶可以以高灵敏度（4 nM或2皮摩尔）检测。还研究了DNA-DNA相互作用。结果表明，序列选择性DNA检测可以实现100 pM（2 nM）的灵敏度。使用MSB来感测这些相互作用的结果验证了基于MSB的感测技术可以实现快速测量（在10秒内），并且适合于检测和定量广泛的生物标志物或分析物。它有可能被应用于各种生物医学应用或诊断分析。
Functionalized magnetic nanoparticles (mNPs) have shown promise in biosensing and other biomedical applications. Here we use functionalized mNPs to develop a highly sensitive, versatile sensing strategy required in practical biological assays and potentially in vivo analysis. We demonstrate a new sensing scheme based on magnetic spectroscopy of nanoparticle Brownian motion (MSB) to quantitatively detect molecular target. MSB uses the harmonics of oscillating mNPs as a metric for the freedom of rotational motion, thus reflecting the bound state of the mNP. The harmonics can be detected in vivo from nanogram quantities of iron within 5 seconds. Using a streptavidin-biotin binding system, we show that the detection limit of the current MSB technique is lower than 150 pM (0.075 picomole), which is much more sensitive than previously reported techniques based on mNP detection. Using mNPs conjugated with two anti-thrombin DNA aptamers, we show that thrombin can be detected with high sensitivity (4 nM or 2 picomole). A DNA-DNA interaction was also investigated. The results demonstrated that sequence selective DNA detection can be achieved with 100 pM (2 nM) sensitivity. The results of using MSB to sense these interactions, verified that the MSB based sensing technique can achieve rapid measurement (within 10 sec), and is suitable for detecting and quantifying a wide range of biomarkers or analytes. It has the potential to be applied in variety of biomedical applications or diagnostic analyses.
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影响因子：
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作者：
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通讯作者：
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