Transcriptome analysis uncovers the diagnostic value of miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma.
Transcriptome analysis uncovers the diagnostic value of miR-192-5p/HNF1A-AS1/VIL1 panel in cervical adenocarcinoma.
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DOI：
10.1038/s41598-020-73523-0
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发表时间：
2020-10-06
影响因子：
4.6
通讯作者：
Lu W
中科院分区：
文献类型：
--
作者：
Xu J;Zou J;Wu L;Lu W
尽管宫颈鳞状细胞癌的发病率有所下降，但宫颈腺癌的发病率却有所上升。然而，我们对宫颈腺癌的认识还很不清楚。进行转录组测序以比较4个宫颈腺癌组织样品与4个正常宫颈组织样品。mRNA、lncRNA和miRNA标记被鉴定以区分宫颈腺癌和正常宫颈。VIL 1、HNF 1A-AS 1、MIR 194 - 2 HG、SSTR 5-AS 1、miR-192- 5 p和miR-194- 5 p在腺癌中的表达均高于正常对照组，差异有统计学意义。受试者工作特征（ROC）曲线分析表明，miR-192- 5 p、HNF 1A-AS 1和VIL 1的组合产生了比任何单一分子更好的性能（AUC = 0.911），并且可以作为宫颈腺癌的潜在生物标志物。值得注意的是，组合模型在宫颈腺癌诊断方面也比TCT测试具有更好的性能。然而，miR-192- 5 p或HNF 1A-AS 1与HPV 16/18 E6或E7之间没有相关性。VIL 1与HPV 18 E7表达呈弱相关。总之，我们的研究已经鉴定了miR-192- 5 p/HNF 1A-AS 1/VIL 1组，其准确区分腺癌和正常宫颈。检测该蛋白对宫颈腺癌的诊断具有重要的临床价值。
Despite the fact that the incidence of cervical squamous cell carcinoma has decreased, there is an increase in the incidence of cervical adenocarcinoma. However, our knowledge on cervical adenocarcinoma is largely unclear. Transcriptome sequencing was conducted to compare 4 cervical adenocarcinoma tissue samples with 4 normal cervical tissue samples. mRNA, lncRNA, and miRNA signatures were identified to discriminate cervical adenocarcinoma from normal cervix. The expression of VIL1, HNF1A-AS1, MIR194-2HG, SSTR5-AS1, miR-192-5p, and miR-194-5p in adenocarcinoma were statistically significantly higher than that in normal control samples. The Receiver Operating Characteristic (ROC) curve analysis indicated that combination of miR-192-5p, HNF1A-AS1, and VIL1 yielded a better performance (AUC = 0.911) than any single molecule -and could serve as potential biomarkers for cervical adenocarcinoma. Of note, the combination model also gave better performance than TCT test for cervical adenocarcinoma diagnosis. However, there was no correlation between miR-192-5p or HNF1A-AS1 and HPV16/18 E6 or E7. VIL1 was weakly correlated with HPV18 E7 expression. In summary, our study has identified miR-192-5p/HNF1A-AS1/VIL1 panel that accurately discriminates adenocarcinoma from normal cervix. Detection of this panel may provide considerable clinical value in the diagnosis of cervical adenocarcinoma.
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作者：
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
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影响因子：
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作者：
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