Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
Radiological assessment of bioengineered bone in a muscle flap for the reconstruction of critical-size mandibular defect.
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DOI：
10.1371/journal.pone.0107403
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
McMahon J
中科院分区：
文献类型：
--
作者：
Al-Fotawei R;Ayoub AF;Heath N;Naudi KB;Tanner KE;Dalby MJ;McMahon J
本研究提出了一个全面的放射学评价骨再生内带蒂肌瓣重建下颌骨缺损的临界尺寸。在10只实验兔的下颌骨上造成20 mm×15 mm的外科缺损。咬肌适合于填充手术缺损，硫酸钙/羟基磷灰石水泥（CERAMENT™）的组合|SPINE SUPPORT）、BMP-7和兔间充质基质细胞（rMSC）注射到肌肉组织内。在手术当天和术后4、8和12周进行影像学评估。在12周时，处死动物，并进行锥形束计算机断层扫描（CBCT）和显微计算机断层扫描（µ-CT）。在临床上，一个清晰的骨组织层被确定为紧密粘附在手术缺损的边缘。放射学检查发现手术缺损内存在零星的不透射线区域。与对侧非手术对照侧相比，不透射线的定量评分估计值为46.6% ±15，不透射线区域的平均体积为63.4% ±20。在手术缺损边缘检测到骨密度高于下颌骨（+35% ±25%）的区域。显微CT分析显示再生骨的骨小梁较薄，比周围的天然骨具有更密集的骨小梁模式。这些发现表明矿化组织的快速沉积速率和肌瓣内新再生骨的积极重塑过程。本研究的新型手术模型具有潜在的临床应用;使用所提出的放射学方案对骨再生进行的评估是描述性的和全面的。本研究的结果证实了局部肌瓣作为局部生物反应器诱导骨形成重建颌面骨缺损的显着潜力。
This study presents a comprehensive radiographic evaluation of bone regeneration within a pedicled muscle flap for the reconstruction of critical size mandibular defect. The surgical defect (20 mm×15 mm) was created in the mandible of ten experimental rabbits. The masseter muscle was adapted to fill the surgical defect, a combination of calcium sulphate/hydroxyapatite cement (CERAMENT™ |SPINE SUPPORT), BMP-7 and rabbit mesenchymal stromal cells (rMSCs) was injected inside the muscle tissue. Radiographic assessment was carried out on the day of surgery and at 4, 8, and 12 weeks postoperatively. At 12 weeks, the animals were sacrificed and cone beam computerized tomography (CBCT) scanning and micro-computed tomography (µ-CT) were carried out. Clinically, a clear layer of bone tissue was identified closely adherent to the border of the surgical defect. Sporadic radio-opaque areas within the surgical defect were detected radiographically. In comparison with the opposite non operated control side, the estimated quantitative scoring of the radio-opacity was 46.6% ±15, the mean volume of the radio-opaque areas was 63.4% ±20. Areas of a bone density higher than that of the mandibular bone (+35% ±25%) were detected at the borders of the surgical defect. The micro-CT analysis revealed thinner trabeculae of the regenerated bone with a more condensed trabecular pattern than the surrounding native bone. These findings suggest a rapid deposition rate of the mineralised tissue and an active remodelling process of the newly regenerated bone within the muscle flap. The novel surgical model of this study has potential clinical application; the assessment of bone regeneration using the presented radiolographic protocol is descriptive and comprehensive. The findings of this research confirm the remarkable potential of local muscle flaps as local bioreactors to induce bone formation for reconstruction of maxillofacial bony defects.
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影响因子：
6.2
作者：
Frost, HM
通讯作者：
Frost, HM
影响因子：
0.9
作者：
Kim, Bong Chul;Yoon, Jung-Hoon;Lee, Jun
通讯作者：
Lee, Jun
影响因子：
3.3
作者：
Lagravere, M. O.;Fang, Y.;Major, P. W.
通讯作者：
Major, P. W.
DOI：
10.1111/j.1749-6632.2002.tb03056.x
发表时间：
2002-01-01
期刊：
REPARATIVE MEDICINE: GROWING TISSUES AND ORGANS
影响因子：
--
作者：
Griffith, LG
通讯作者：
Griffith, LG
影响因子：
4.1
作者：
Kothari, M;Keaveny, TM;Majumdar, S
通讯作者：
Majumdar, S