Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity.
Quantitative analysis of the tomato nuclear proteome during Phytophthora capsici infection unveils regulators of immunity.
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DOI：
10.1111/nph.14540
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发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Huitema E
中科院分区：
文献类型：
--
作者：
Howden AJM;Stam R;Martinez Heredia V;Motion GB;Ten Have S;Hodge K;Marques Monteiro Amaro TM;Huitema E
植物-病原体相互作用是由宿主和微生物编码因子的相互作用驱动的复杂关联。由于在植物细胞核中发现了分泌的病原体蛋白（效应物）和免疫信号成分，该隔室是指定易感性的战场。我们假设，通过定义感染期间核蛋白质组的变化，我们可以确定免疫或易感性所需的重要成分。我们通过记录卵菌病原体辣椒疫霉菌感染期间番茄（Solanum lycopersicum）核蛋白质组的动态变化来验证这一假设。我们从感染和未感染组织中富集细胞核，并定量评估细胞核蛋白质组的变化。然后，我们通过功能测定测试了候选调节剂在免疫中的作用。我们证明了宿主细胞核蛋白质组在辣椒疫霉感染过程中发生动态变化。我们观察到，已知的核免疫因子的差异表达，并根据这一观察结果，选择了一组候选调节剂，我们成功地牵连到辣椒疫霉的免疫。我们的工作证明了一种强大的策略，可以快速洞察支撑抗性等复杂作物性状的重要核过程。我们已经确定了一个大的候选核因子，可能会支持免疫农作物病原体。
Plant–pathogen interactions are complex associations driven by the interplay of host and microbe‐encoded factors. With secreted pathogen proteins (effectors) and immune signalling components found in the plant nucleus, this compartment is a battleground where susceptibility is specified. We hypothesized that, by defining changes in the nuclear proteome during infection, we can pinpoint vital components required for immunity or susceptibility. We tested this hypothesis by documenting dynamic changes in the tomato (Solanum lycopersicum) nuclear proteome during infection by the oomycete pathogen Phytophthora capsici. We enriched nuclei from infected and noninfected tissues and quantitatively assessed changes in the nuclear proteome. We then tested the role of candidate regulators in immunity through functional assays. We demonstrated that the host nuclear proteome dynamically changes during P. capsici infection. We observed that known nuclear immunity factors were differentially expressed and, based on this observation, selected a set of candidate regulators that we successfully implicated in immunity to P. capsici. Our work exemplifies a powerful strategy to gain rapid insight into important nuclear processes that underpin complex crop traits such as resistance. We have identified a large set of candidate nuclear factors that may underpin immunity to pathogens in crops.
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