Expression pattern of thymosin beta 4 in the adult human liver.
Expression pattern of thymosin beta 4 in the adult human liver.
复制标题
成人肝脏中胸腺素β4的表达模式。
DOI：
10.4081/ejh.2011.e25
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Faa G
中科院分区：
文献类型：
--
作者：
Nemolato S;Van Eyken P;Cabras T;Cau F;Fanari MU;Locci A;Fanni D;Gerosa C;Messana I;Castagnola M;Faa G
胸腺素β 4（Thymosin beta 4，Tβ4）是β-胸腺素家族的一员，它参与G-肌动蛋白的聚合，并参与许多重要的生物学过程，包括细胞凋亡、细胞迁移、血管生成和纤维化。先前在新生儿肝脏中的研究未显示在宫内生活期间对Tβ4有任何显著反应性。本研究旨在通过免疫组织化学方法研究Tβ4在成人正常肝脏中的表达。使用抗T β4商业抗体对35份人类肝脏样本进行免疫染色，其中包括11份肝穿刺活检标本和24份尸检时获得的肝脏标本，其中在组织学检查中未检测到病理变化。正常成人肝细胞内均检测到Tβ4。在绝大多数病例中，Tβ4表达呈明显的带状分布。免疫染色优先在3区检测到，而在门静脉周围肝细胞（1区）检测到轻微程度的反应。高倍镜下，Tβ4反应颗粒主要位于肝细胞胆极。在具有强免疫染色的病例中，甚至肝细胞的核周区和窦极也出现Tβ4免疫反应性。目前的工作首次证明了成年人肝脏中Tβ4的强烈弥漫性表达，并将肝细胞添加到能够在成年期合成大量Tβ4的人类细胞列表中。此外，Tβ4应添加到以带状表达模式为特征的肝蛋白中，从终末静脉到肝腺泡的门静脉周围区域呈下降梯度。确定这种肽在细胞内和细胞外、生理学和不同肝脏疾病中所发挥的密切作用，是未来重点研究Tβ4的一个重大挑战。
Thymosin beta-4 (Tβ4) is a member of beta-thymosins, a family of small peptides involved in polymerization of G-actin, and in many critical biological processes including apoptosis, cell migration, angiogenesis, and fibrosis. Previous studies in the newborn liver did not reveal any significant reactivity for Tβ4 during the intrauterine life. The aim of the present study was to investigate by immunohistochemistry Tβ4 expression in the adult normal liver. Thirty-five human liver samples, including 11 needle liver biopsies and 24 liver specimens obtained at autopsy, in which no pathological change was detected at the histological examination, were immunostained utilizing an anti-Tβ4 commercial antibody. Tβ4 was detected in the hepatocytes of all adult normal livers examined. A zonation of Tβ4 expression was evident in the vast majority of cases. Immunostaining was preferentially detected in zone 3, while a minor degree of reactivity was detected in periportal hepatocytes (zone 1). At higher power, Tβ4-reactive granules appeared mainly localized at the biliary pole of hepatocytes. In cases with a strong immunostaining, even perinuclear areas and the sinusoidal pole of hepatocytes appeared interested by immunoreactivity for Tβ4. The current work first evidences a strong diffuse expression of Tβ4 in the adult human liver, and adds hepatocytes to the list of human cells able to synthesize large amounts of Tβ4 in adulthood. Moreover, Tβ4 should be added to the liver proteins characterized by a zonate expression pattern, in a descending gradient from the terminal vein to the periportal areas of the liver acinus. Identifying the intimate role played by this peptide intracellularly and extracellularly, in physiology and in different liver diseases, is a major challenge for future research focusing on Tβ4.
登录
查看更多内容
DOI：
10.1196/annals.1415.046
发表时间：
2007-01-01
期刊：
THYMOSINS IN HEALTH AND DISEASE: FIRST INTERNATIONAL SYMPOSIUM
影响因子：
--
作者：
Badamchian, Mahnaz;Damavandy, Ali A.;Goldstein, Allan L.
通讯作者：
Goldstein, Allan L.
DOI：
10.1073/pnas.53.4.812
发表时间：
1965-01-01
影响因子：
11.1
作者：
KLEIN, JJ;GOLDSTEIN, AL;WHITE, A
通讯作者：
WHITE, A
影响因子：
3.9
作者：
Huang, Wei Qi;Wang, Bao He;Wang, Qi Ru
通讯作者：
Wang, Qi Ru
胎儿和成人胃肠道中的不同胸腺素β4免疫反应性。
影响因子：
3.7
作者：
Nemolato, Sonia;Cabras, Tiziana;Cau, Flaviana;Fanari, Mattia Umberto;Fanni, Daniela;Manconi, Barbara;Messana, Irene;Castagnola, Massimo;Faa, Gavino
通讯作者：
Faa, Gavino
DOI：
10.1196/annals.1415.035
发表时间：
2007-01-01
期刊：
THYMOSINS IN HEALTH AND DISEASE: FIRST INTERNATIONAL SYMPOSIUM
影响因子：
--
作者：
Barnaeva, Elena;Nadezhda, Agladze;Rojkind, Marcos
通讯作者：
Rojkind, Marcos