Interaction of brassinosteroids and polyamines enhances copper stress tolerance in raphanus sativus.
Interaction of brassinosteroids and polyamines enhances copper stress tolerance in raphanus sativus.
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DOI：
10.1093/jxb/ers219
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发表时间：
2012-09
影响因子：
6.9
通讯作者：
Tran LS
中科院分区：
文献类型：
--
作者：
Choudhary SP;Oral HV;Bhardwaj R;Yu JQ;Tran LS
油菜素内酯（BRs）和多胺（PAs）调节对非生物胁迫的各种响应，但它们在受铜（Cu）毒害水平的植物中对铜稳态调节的作用尚不清楚。本研究分析了外源施加的BRs和PAs对暴露于有毒浓度铜的萝卜（Raphanus sativus）植株的影响。24 - 表油菜素内酯（EBR，一种活性BR）和亚精胺（Spd，一种活性PA）对萝卜植株基因表达和生理的相互作用导致对铜胁迫的耐受性增强。结果表明，EBR和Spd的联合施用调节了编码多胺酶的基因以及影响吲哚 - 3 - 乙酸（IAA）和脱落酸（ABA）代谢的基因的表达，从而增强了对铜胁迫的耐受性。参与铜稳态的基因表达改变似乎是EBR和Spd缓解萝卜铜胁迫的主要作用。离子泄漏、过氧化氢体内成像、彗星试验以及对铜敏感酵母菌株耐受性的提高，进一步证明了EBR和Spd显著提高铜耐受性的能力。研究表明，EBR和Spd的共同施用是植物中铜解毒和维持铜稳态的有效方法。因此，应探索这些化合物在农业生产系统中的应用。
Brassinosteroids (BRs) and polyamines (PAs) regulate various responses to abiotic stress, but their involvement in the regulation of copper (Cu) homeostasis in plants exposed to toxic levels of Cu is poorly understood. This study provides an analysis of the effects of exogenously applied BRs and PAs on radish (Raphanus sativus) plants exposed to toxic concentrations of Cu. The interaction of 24-epibrassinolide (EBR, an active BR) and spermidine (Spd, an active PA) on gene expression and the physiology of radish plants resulted in enhanced tolerance to Cu stress. Results indicated that the combined application of EBR and Spd modulated the expression of genes encoding PA enzymes and genes that impact the metabolism of indole-3-acetic acid (IAA) and abscisic acid (ABA) resulting in enhanced Cu stress tolerance. Altered expression of genes implicated in Cu homeostasis appeared to be the main effect of EBR and Spd leading to Cu stress alleviation in radish. Ion leakage, in vivo imaging of H2O2, comet assay, and improved tolerance of Cu-sensitive yeast strains provided further evidence for the ability of EBR and Spd to improve Cu tolerance significantly. The study indicates that co-application of EBR and Spd is an effective approach for Cu detoxification and the maintenance of Cu homeostasis in plants. Therefore, the use of these compounds in agricultural production systems should be explored.
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