Claudin-7 is highly expressed in chromophobe renal cell carcinoma and renal oncocytoma.
Claudin-7 is highly expressed in chromophobe renal cell carcinoma and renal oncocytoma.
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DOI：
10.3346/jkms.2007.22.2.305
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发表时间：
2007-04
影响因子：
4.5
通讯作者：
Choi C
中科院分区：
文献类型：
--
作者：
Choi YD;Kim KS;Ryu S;Park Y;Cho NH;Rha SH;Jang JJ;Ro JY;Juhng SW;Choi C
Claudin-7最近被认为是远端肾单位的标志物。我们测试了Claudin-7的表达可以作为起源于远端肾单位的肾脏肿瘤的标记的可能性。应用免疫组织化学方法检测了肾透明细胞癌S、乳头状肾细胞癌29例、嫌色肾细胞癌20例和肾嗜酸细胞瘤11例中Claudin-7和Parvalbrain的表达。此外，还进行了claudin-7的甲基化特异性聚合酶链式反应(MSP)。在透明细胞型、乳头状型、嫌色型、嗜色型肾细胞癌中分别为3.4%、7.8%、34.5%、31.0%、95.0%、80.0%、72.7%、81.8%。Claudin-7在不同亚型中诊断嫌色肾癌的敏感性和特异性分别为95.0%和92.3%。小白蛋白分别为80.0%和88.9%。Claudin-7在嫌色肾细胞癌中主要呈弥漫性表达，在嗜酸细胞瘤中呈灶性或弥漫性表达。MSP检查的所有病例均显示Claudin-7的非甲基化启动子的存在，而与Claudin-7的免疫反应无关。启动子的高甲基化可能不是其在肾细胞癌中表达缺失的潜在机制。Claudin-7可作为诊断嫌色肾细胞癌和嗜酸细胞瘤的有用标志物。
Claudin-7 has recently been suggested to be a distal nephron marker. We tested the possibility that expression of claudin-7 could be used as a marker of renal tumors originating from the distal nephron. We examined the immunohistochemical expression of claudin-7 and parvalbumin in 239 renal tumors, including 179 clear cell renal cell carcinoma (RCC)s, 29 papillary RCCs, 20 chromophobe RCCs, and 11 renal oncocytomas. In addition, the methylation specific-PCR (MSP) of claudin-7 was performed. Claudin-7 and parvalbumin immunostains were positive in 3.4%, 7.8% of clear cell RCCs, 34.5%, 31.0% of papillary RCCs, 95.0%, 80.0% of chromophobe RCCs, and 72.7%, 81.8% of renal oncocytomas, respectively. The sensitivity and specificity of claudin-7 in diagnosing chromophobe RCC among subtypes of RCC were 95.0% and 92.3%. Those of parvalbumin were 80.0% and 88.9%. The expression pattern of claudin-7 was mostly diffuse in chromophobe RCC and was either focal or diffuse in oncocytoma. All of the cases examined in the MSP revealed the presence of unmethylated promoter of claudin-7 without regard to claudin-7 immunoreactivity. Hypermethylation of the promoter might not be the underlying mechanism for loss of its expression in RCC. Claudin-7 can be used as a useful diagnostic marker in diagnosing chromophobe RCC and oncocytoma.
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影响因子：
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作者：
Martignoni, G;Pea, M;Bonetti, F
通讯作者：
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