Apple pectin, a dietary fiber, ameliorates myocardial injury by inhibiting apoptosis in a rat model of ischemia/reperfusion.
Apple pectin, a dietary fiber, ameliorates myocardial injury by inhibiting apoptosis in a rat model of ischemia/reperfusion.
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DOI：
10.4162/nrp.2014.8.4.391
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发表时间：
2014-08
影响因子：
2.4
通讯作者：
Lee J
中科院分区：
文献类型：
--
作者：
Lim SH;Kim MY;Lee J
由于冠状动脉闭塞导致的心肌细胞死亡导致心肌梗死，这是冠心病（CHD）的一个子集。已知膳食纤维与降低CHD风险相关，其潜在机制被认为是通过改善风险因素来延迟闭塞的发生。在这项研究中，我们测试了一个假设，即膳食纤维的有益作用可能来自于保护心肌细胞免受动脉闭塞后表现出的缺血性损伤。大鼠灌胃苹果果胶（AP）（10、40、100和400 mg/kg/d）3 d后，结扎冠状动脉左前降支30 min，再灌注3 h，造成心肌缺血损伤。分别用伊文思蓝染色和氯化三苯基四氮唑（TTC）染色评价危险区和梗死区。TUNEL法检测心肌细胞凋亡程度。通过免疫组化评估切割的caspase-3、Bcl-2和Bax的水平。与对照组相比，补充AP（100和400 mg/kg/天）导致AP治疗组的梗死面积（IS）（梗死面积与风险面积的比值）分别显著降低21.9%和22.4%。IS中的这种衰减显示与参与凋亡级联反应的生物标志物的改善相关：凋亡细胞减少，半胱天冬酶原-3转化为半胱天冬酶-3的抑制，以及Bcl-2/Bax比率（细胞命运的决定因素）的增加。结果表明，补充AP通过抑制细胞凋亡而改善心肌梗死。因此，目前的研究表明，膳食纤维的摄入不仅通过阻断导致闭塞的步骤，而且还通过保护动脉闭塞引起的缺血性损伤来降低CHD的风险。
Myocardial cell death due to occlusion of the coronary arteries leads to myocardial infarction, a subset of coronary heart disease (CHD). Dietary fiber is known to be associated with a reduced risk of CHD, the underlying mechanisms of which were suggested to delay the onset of occlusion by ameliorating risk factors. In this study, we tested a hypothesis that a beneficial role of dietary fiber could arise from protection of myocardial cells against ischemic injury, manifested after occlusion of the arteries. Three days after rats were fed apple pectin (AP) (with 10, 40, 100, and 400 mg/kg/day), myocardial ischemic injury was induced by 30 min-ligation of the left anterior descending coronary artery, followed by 3 hr-reperfusion. The area at risk and infarct area were evaluated using Evans blue dye and 2,3,5-triphenyltetrazolium chloride (TTC) staining, respectively. DNA nicks reflecting the extent of myocardial apoptosis were assessed by TUNEL assay. Levels of cleaved caspase-3, Bcl-2, and Bax were assessed by immunohistochemistry. Supplementation of AP (with 100 and 400 mg/kg/day) resulted in significantly attenuated infarct size (IS) (ratio of infarct area to area at risk) by 21.9 and 22.4%, respectively, in the AP-treated group, compared with that in the control group. This attenuation in IS showed correlation with improvement in biomarkers involved in the apoptotic cascades: reduction of apoptotic cells, inhibition of conversion of procaspase-3 to caspase-3, and increase of Bcl-2/Bax ratio, a determinant of cell fate. The findings indicate that supplementation of AP results in amelioration of myocardial infarction by inhibition of apoptosis. Thus, the current study suggests that intake of dietary fiber reduces the risk of CHD, not only by blocking steps leading to occlusion, but also by protecting against ischemic injury caused by occlusion of the arteries.
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影响因子：
--
作者：
Lim SH;Lee J
通讯作者：
Lee J
DOI：
10.3839/jksabc.2010.088
发表时间：
2010-10-01
影响因子：
--
作者：
Lim, Sun Ha;Song, Kyung-Sik;Lee, Jongwon
通讯作者：
Lee, Jongwon
影响因子：
30.3
作者：
Fischbach MA;Sonnenburg JL
通讯作者：
Sonnenburg JL
影响因子：
56.9
作者：
Lakhani, SA;Masud, A;Flavell, RA
通讯作者：
Flavell, RA
影响因子：
4.2
作者：
Aprikian, O;Duclos, V;Demigné, C
通讯作者：
Demigné, C