Proposed three-phenylalanine motif involved in magnetoreception signalling of an Actinopterygii protein expressed in mammalian cells.
Proposed three-phenylalanine motif involved in magnetoreception signalling of an Actinopterygii protein expressed in mammalian cells.
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作者：
在细胞和分子水平上研究磁感受器对磁场的感受和响应是一个相对较新的研究领域。动物的磁感受机制似乎是从不同的起源进化而来的，因此，关于其机制的许多问题仍然是开放的。在这里，我们提出了新的信息，电磁感知基因（EPG）从Kryptopterus vitreolus，可能作为基础的一部分，以了解和应用磁感受。使用HaloTag与荧光配体和磷脂酰肌醇特异性磷脂酶C偶联，我们表明EPG通过糖基磷脂酰肌醇锚与膜相关。利用EPG增加细胞内钙离子的功能，建立了GCaMP 6m测定法，观察EPG的功能，并与其同源蛋白的功能进行比较。还揭示了EPG依赖于三个苯丙氨酸残基的基序来功能稳定地交换这些残基，使用定点诱变导致EPG中的功能丧失。这些信息不仅扩展了我们目前对磁感受的理解，而且可以提供一个基础和模板，以继续在新兴领域中表征和发现更多。
Studies at the cellular and molecular level of magnetoreception—sensing and responding to magnetic fields—are a relatively new research area. It appears that different mechanisms of magnetoreception in animals evolved from different origins, and, therefore, many questions about its mechanisms remain left open. Here we present new information regarding the Electromagnetic Perceptive Gene (EPG) from Kryptopterus vitreolus that may serve as part of the foundation to understanding and applying magnetoreception. Using HaloTag coupled with fluorescent ligands and phosphatidylinositol specific phospholipase C we show that EPG is associated with the membrane via glycosylphosphatidylinositol anchor. EPG's function of increasing intracellular calcium was also used to generate an assay using GCaMP6m to observe the function of EPG and to compare its function with that of homologous proteins. It was also revealed that EPG relies on a motif of three phenylalanine residues to function—stably swapping these residues using site directed mutagenesis resulted in a loss of function in EPG. This information not only expands upon our current understanding of magnetoreception but may provide a foundation and template to continue characterizing and discovering more within the emerging field.
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影响因子：
16.6
作者：
Myklatun A;Lauri A;Eder SHK;Cappetta M;Shcherbakov D;Wurst W;Winklhofer M;Westmeyer GG
通讯作者：
Westmeyer GG
影响因子：
4.5
作者：
Loughner CL;Bruford EA;McAndrews MS;Delp EE;Swamynathan S;Swamynathan SK
通讯作者：
Swamynathan SK
DOI：
10.1126/science.aat7113
发表时间：
2018-09-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Herberg S;Gert KR;Schleiffer A;Pauli A
通讯作者：
Pauli A
影响因子：
3.4
作者：
Tian R;Losilla M;Lu Y;Yang G;Zakon H
通讯作者：
Zakon H
影响因子：
5.5
作者：
Hwang, Jangsun;Choi, Yonghyun;Choi, Jonghoon
通讯作者：
Choi, Jonghoon