Differences in Gut Microbiome Composition and Antibiotic Resistance Gene Distribution between Chinese and Pakistani University Students from a Common Peer Group.
Differences in Gut Microbiome Composition and Antibiotic Resistance Gene Distribution between Chinese and Pakistani University Students from a Common Peer Group.
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DOI：
10.3390/microorganisms9061152
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发表时间：
2021-05-27
期刊：
影响因子：
4.5
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Feng T;Hilal MG;Wang Y;Zhou R;Yu Q;Qu J;Li H
肠道微生物组在人类健康中起着重要的功能作用，也受到许多因素的影响。然而，很少有研究集中在运动干预下的肠道微生物组。此外，肠道微生物组携带的抗生素耐药基因（ARG）可能会不断对人类健康构成威胁。本研究采用16S rRNA基因测序和高通量定量PCR技术对中国和巴基斯坦参与者的ARG和微生物组进行了研究。在观察期间，运动对12名受试者的肠道微生物组没有影响，而不同民族的肠道微生物组分布不同。总体而言，参与者肠道中的主要微生物门是拟杆菌门、厚壁菌门和变形菌门。其中普雷沃氏菌属和小类杆菌属在巴基斯坦人群中的数量较多，拟杆菌属和粪杆菌属在中国人群中的数量较多。中国人的微生物多样性高于巴基斯坦人。此外，中国人和巴基斯坦人的微生物群落结构也存在差异。对于ARG，所有检测到的ARG的分布在每个时间点都不明显。在这些ARG中，floR在中国和巴基斯坦参与者中的分布不同，一些ARG如tetQ和sul2与几种优势微生物组正相关，特别是Bacteroidota和Firmicutes细菌，它们不会随时间波动。
Gut microbiomes play important functional roles in human health and are also affected by many factors. However, few studies concentrate on gut microbiomes under exercise intervention. Additionally, antibiotic resistance genes (ARGs) carried by gut microbiomes may constantly pose a threat to human health. Here, ARGs and microbiomes of Chinese and Pakistanis participants were investigated using 16S rRNA gene sequencing and high-throughput quantitative PCR techniques. The exercise had no impact on gut microbiomes in the 12 individuals investigated during the observation period, while the different distribution of gut microbiomes was found in distinct nationalities. Overall, the dominant microbial phyla in the participants’ gut were Bacteroidota, Firmicutes and Proteobacteria. Some genera such as Prevotella and Dialister were more abundant in Pakistani participants and some other genera such as Bacteroides and Faecalibacterium were more abundant in Chinese participants. The microbial diversity in Chinese was higher than that in Pakistanis. Furthermore, microbial community structures were also different between Chinese and Pakistanis. For ARGs, the distribution of all detected ARGs is not distinct at each time point. Among these ARGs, floR was distributed differently in Chinese and Pakistani participants, and some ARGs such as tetQ and sul2 are positively correlated with several dominant microbiomes, particularly Bacteroidota and Firmicutes bacteria that did not fluctuate over time.
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影响因子：
6.7
作者：
Brown RL;Larkinson MLY;Clarke TB
通讯作者：
Clarke TB
影响因子：
15.5
作者：
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通讯作者：
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DOI：
10.1016/j.ijantimicag.2008.07.029
发表时间：
2009-02-01
影响因子：
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影响因子：
29.4
作者：
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通讯作者：
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