Taxonomy and Functional Diversity in the Fecal Microbiome of Beef Cattle Reared in Brazilian Traditional and Semi-Intensive Production Systems.
Taxonomy and Functional Diversity in the Fecal Microbiome of Beef Cattle Reared in Brazilian Traditional and Semi-Intensive Production Systems.
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DOI：
10.3389/fmicb.2021.768480
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发表时间：
2021
影响因子：
5.2
通讯作者：
Louvandini H
中科院分区：
文献类型：
--
作者：
Corrêa PS;Jimenez CR;Mendes LW;Rymer C;Ray P;Gerdes L;da Silva VO;De Nadai Fernandes EA;Abdalla AL;Louvandini H
牛肉生产对巴西经济的重要性和地球仪对动物蛋白的需求不断增长，需要改进牛肉生产系统。虽然大多数注意力都集中在调节瘤胃微生物组以提高反刍动物产量上，但下消化道微生物组在宿主健康和营养中的作用仍然相对未被探索。这项工作的目的是调查的分类和功能的变化，巴西肉牛的粪便微生物饲养在两个不同的生产系统，使用宏基因组学的方法。来自6个农场的60头雄性肉牛，代表半集约化（I，n = 2）和传统（T，n = 4）巴西牛肉生产系统，入组本研究。鸟枪测序用于表征从每只动物收集的粪便样品中微生物组的分类和功能组成以及多样性。分析粪便样品中的铜（Cu）、铅（Pb）、氮（N）、磷（P）、硒（Se）和锌（Zn）以及碳（13 C）和氮（15 N）的稳定同位素。与T系统相比，I系统肉牛粪便的功能多样性更大，分类多样性更低，粪便微生物组受到牛肉生产系统的影响。与T系统相比，I系统中肉牛粪便中N，P和Zn的浓度较高，并且与I系统中粪便微生物组的分类和功能特征相关，表明粪便营养物质在形成系统特异性微生物组中的作用。半集约化管理实践导致肉牛粪便微生物组更加复杂，但联系较少。I系统肉牛粪便中的微生物群落的特征在于更丰富的有益细菌（厚壁菌门和丁酸盐产生菌家族毛螺菌科和厌氧柄菌属、布劳特氏菌属、丁酸弧菌属、真杆菌属、罗斯拜瑞氏菌属和瘤胃球菌属）。此外，粪便中与免疫系统、营养代谢和能量生产相关的微生物基因丰度在I系统饲养的肉牛中高于T系统饲养的肉牛。目前的研究结果表明，半集约化管理实践可以通过增加有益细菌和功能基因的丰度，促进肉牛粪便微生物组的健康和高效发展。
The importance of beef production for economy of Brazil and the growing demand for animal protein across the globe warrant an improvement in the beef production system. Although most attention has been on modulation of the rumen microbiome to improve ruminant production, the role of the lower gut microbiome in host health and nutrition remains relatively unexplored. This work aimed to investigate the taxonomy and functional variations in the fecal microbiome of Brazilian beef cattle reared in two different production systems using a metagenomic approach. Sixty male beef cattle from six farms representing semi-intensive (I, n = 2) and traditional (T, n = 4) Brazilian beef production systems were enrolled in the study. Shotgun sequencing was used to characterize taxonomic and functional composition and diversity of the microbiome in fecal samples collected from each animal. Fecal samples were analyzed for copper (Cu), lead (Pb), nitrogen (N), phosphorous (P), selenium (Se), and zinc (Zn) and stable isotopes of carbon (13C) and nitrogen (15N). The fecal microbiome was influenced by the beef production systems with greater functional and lower taxonomic diversity in beef cattle feces from I systems compared with that from T systems. The concentration of N, P, and Zn was higher in beef cattle feces from I systems compared with that from T systems and was associated with taxonomic and functional profile of fecal microbiome in I system, suggesting the role of fecal nutrients in shaping system-specific microbiome. Semi-intensive management practices led to a more complex but less connected fecal microbiome in beef cattle. The microbial community in beef cattle feces from I systems was characterized by greater abundance of beneficial bacteria (phylum Firmicutes and butyrate-producing bacteria family Lachnospiraceae and genera Anaerostipes, Blautia, Butyrivibrio, Eubacterium, Roseburia, and Ruminococcus). In addition, the fecal abundance of microbial genes related to immune system, nutrient metabolism, and energy production was greater in beef cattle raised under I systems compared with that under T systems. Findings of the current study suggest that semi-intensive management practices could facilitate the development of a healthier and more efficient fecal microbiome in beef cattle by driving an increase in the abundance of beneficial bacteria and functional genes.
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影响因子：
7.7
作者：
Cani, Patrice D.;Amar, Jacques;Burcelin, Remy
通讯作者：
Burcelin, Remy
影响因子：
15.5
作者：
Auffret MD;Dewhurst RJ;Duthie CA;Rooke JA;John Wallace R;Freeman TC;Stewart R;Watson M;Roehe R
通讯作者：
Roehe R
影响因子：
4.6
作者：
Azad, E.;Derakhshani, H.;Khafipour, E.
通讯作者：
Khafipour, E.
影响因子：
3.7
作者：
de Faria Maciel, Isabella Cristina;Barbosa, Fabiano Alvim;Quintao Lana, Angela Maria
通讯作者：
Quintao Lana, Angela Maria
影响因子：
3.3
作者：
Freetly, Harvey C;Dickey, Aaron;Wells, James E
通讯作者：
Wells, James E