Germ cell transplantation using sexually competent fish: an approach for rapid propagation of endangered and valuable germlines.
Germ cell transplantation using sexually competent fish: an approach for rapid propagation of endangered and valuable germlines.
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DOI：
10.1371/journal.pone.0006132
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发表时间：
2009-07-02
期刊：
影响因子：
3.7
通讯作者：
Strüssmann CA
中科院分区：
文献类型：
--
作者：
Majhi SK;Hattori RS;Yokota M;Watanabe S;Strüssmann CA
将生殖细胞移植到成年受体性腺中是一种在动物育种和保护珍贵和/或濒危物种中具有广泛应用的工具，它还为涉及生殖细胞（GC）增殖和分化的基础研究提供了手段。在这里，我们描述了一个工作模型的建立异种生殖细胞移植（GCT）在性能力的鱼类。将从一种银汉鱼（Atherinopsidae）的幼鱼中分离的精原细胞移植到性成熟的巴塔哥尼亚鱼（Patagonian pejerrey O. hatcheri，其已经通过白消安（40 mg/kg）和高水温（25 ° C）处理的组合部分耗尽内源GC。对供体细胞行为的观察表明，移植的精原细胞能使受体性腺分化，并在GCT后6个月内恢复精子发生。通过PCR在20%的替代O中证实了供体来源的配子的存在。hatcheri父亲在6个月，并与O。bonariensis的母体产生了杂种和纯O. Bonariensis，供体来源的生殖系传播率为1.2 - 13.3%。这些研究结果表明，精原细胞移植到性能力的鱼，可以大大缩短供体来源的配子和后代的生产时间，并可能发挥重要作用，在种系保存和繁殖的珍贵和/或濒危鱼类物种。
The transplantation of germ cells into adult recipient gonads is a tool with wide applications in animal breeding and conservation of valuable and/or endangered species; it also provides a means for basic studies involving germ cell (GC) proliferation and differentiation. Here we describe the establishment of a working model for xenogeneic germ cell transplantation (GCT) in sexually competent fish. Spermatogonial cells isolated from juveniles of one species, the pejerrey Odontesthes bonariensis (Atherinopsidae), were surgically transplanted into the gonads of sexually mature Patagonian pejerrey O. hatcheri, which have been partially depleted of endogenous GCs by a combination of Busulfan (40 mg/kg) and high water temperature (25°C) treatments. The observation of the donor cells' behavior showed that transplanted spermatogonial cells were able to recolonize the recipients' gonads and resume spermatogenesis within 6 months from the GCT. The presence of donor-derived gametes was confirmed by PCR in 20% of the surrogate O. hatcheri fathers at 6 months and crosses with O. bonariensis mothers produced hybrids and pure O. bonariensis, with donor-derived germline transmission rates of 1.2–13.3%. These findings indicate that transplantation of spermatogonial cells into sexually competent fish can shorten considerably the production time of donor-derived gametes and offspring and could play a vital role in germline conservation and propagation of valued and/or endangered fish species.
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影响因子：
2
作者：
Somoza, Gustavo M.;Miranda, Leandro A.;Strussmann, Carlos A.
通讯作者：
Strussmann, Carlos A.
影响因子：
2
作者：
Strussmann, CA;Akaba, T;Takashima, F
通讯作者：
Takashima, F
影响因子：
4.5
作者：
Arai, K
通讯作者：
Arai, K
DOI：
10.1002/jez.a.197
发表时间：
2005-08-01
影响因子：
2.8
作者：
Bouma, GJ;Cloud, JG;Nagler, JJ
通讯作者：
Nagler, JJ
影响因子：
3.6
作者：
Brinster, CJ;Ryu, BY;Orwig, KE
通讯作者：
Orwig, KE