Instability of self-assembled monolayers as a model material system for macrophage/FBGC cellular behavior.
Instability of self-assembled monolayers as a model material system for macrophage/FBGC cellular behavior.
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DOI：
10.1002/jbm.a.31660
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发表时间：
2008-07
影响因子：
4.9
通讯作者：
Anderson, James M.
中科院分区：
文献类型：
--
作者：
Jones, Jacqueline A.;Qin, L. Abby;Meyerson, Howard;Kwon, Il Keun;Matsuda, Takehisa;Anderson, James M.
设计用于呈现均匀表面化学的新型自组装单分子层（SAMs）被用于进一步研究材料表面化学依赖性巨噬细胞和异物巨细胞（FBGC）行为，包括巨噬细胞粘附、融合和凋亡。接触角分析表明，在37 ℃或干燥的室温条件下，在无血清培养基中孵育后，-CH 3和-COOH终止SAM表面不稳定。进一步的分析表明，-CH 3封端的SAM表面在2小时内迅速降解，并且在37 ° C下在无血清培养基（SFM）中孵育24小时内损失足够的SAM单元以与金（Au）对照表面相当。在37 ℃的SFM中孵育5d后，-COOH封端的SAM表面的接触角显著增加。AFM分析证实了-CH 3终止的SAM分子从表面上的解吸增加的粗糙度和显着的峰和谷的外观在2小时内。-COOH封端的SAM表面的厚度的减小也表明分子随时间的解吸。在接触角或AFM分析中没有观察到显着的变化-OH终止SAM表面。与其他表面相比，细胞粘附在Au对照和-CH 3终止的SAM表面上下降得更快。然而，到第10天，细胞粘附、融合和凋亡在所有SAM表面和Au对照上相当。这些研究表明，SAM表面可能不适合长期的研究，其中材料依赖的性能进行调查。
Novel self-assembled monolayers (SAMs) designed to present homogenous surface chemistries were utilized to further investigate the material surface chemistry dependent macrophage and foreign body giant cell (FBGC) behaviors including macrophage adhesion, fusion, and apoptosis. Contact angle analysis revealed instabilities in the –CH3 and –COOH terminate SAM surfaces upon incubation in serum-free media at 37oC or under dry, room temperatureconditions. Further analysis indicated that the –CH3 terminated SAM surface degraded rapidly within 2 hours and loss of sufficient SAM units to be comparable to the gold (Au) control surface within 24 hours of incubation in serum-free media (SFM) at 37oC. After 5days of incubation in SFM at 37oC, the contact angles for the –COOH terminated SAMsurfaces increased markedly. AFM analysis confirmed the desorption of –CH3 terminated SAM molecules from the surface with increased roughness and marked appearance of peaks andvalleys within 2 hours. A decrease in the thickness of the –COOH terminated SAM surface also suggests molecular desorption over time. No significant changes in contact angle or AFM analyses were observed on the –OH terminated SAM surfaces. Cellular adhesion decreased morerapidly on the Au control and –CH3 terminated SAM surfaces in comparison to the other surfaces. However by day 10, cellular adhesion, fusion, and apoptosis were comparable on all SAM surfaces and the Au control . These studies suggest that SAM surfaces may not be suitable for long-term studies where material dependent properties are investigated.
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影响因子：
14
作者：
Källtorp, M;Oblogina, S;Thomsen, P
通讯作者：
Thomsen, P
DOI：
10.1023/a:1026394431100
发表时间：
2003-11-01
影响因子：
3.7
作者：
Martin, MCL;Naeemi, E;Barbosa, MA
通讯作者：
Barbosa, MA
DOI：
10.1073/pnas.162124199
发表时间：
2002-08-06
影响因子：
11.1
作者：
Brodbeck, WG;Patel, J;Anderson, JM
通讯作者：
Anderson, JM
DOI：
10.1002/jbm.10052
发表时间：
2002-06-15
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Latour, RA;Rini, CJ
通讯作者：
Rini, CJ
DOI：
10.1073/pnas.0408954102
发表时间：
2005-01-25
影响因子：
11.1
作者：
Jiang, XY;Bruzewicz, DA;Whitesides, GM
通讯作者：
Whitesides, GM