Feasibility and preliminary accuracy of high-resolution imaging of the liver and pancreas using FNA compatible microendoscopy (with video).
Feasibility and preliminary accuracy of high-resolution imaging of the liver and pancreas using FNA compatible microendoscopy (with video).
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DOI：
10.1016/j.gie.2012.04.445
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发表时间：
2012-08
影响因子：
7.7
通讯作者：
Kim MK
中科院分区：
文献类型：
--
作者：
Regunathan R;Woo J;Pierce MC;Polydorides AD;Raoufi M;Roayaie S;Schwartz M;Labow D;Shin D;Suzuki R;Bhutani MS;Coghlan LG;Richards-Kortum R;Anandasabapathy S;Kim MK
内镜下超声引导下细针穿刺（EUS-FNA）是为数不多的可以从肝脏和胰腺中获取细胞和组织的技术之一。然而，该技术仍然容易受到样品质量差和采样误差的影响。评估高分辨率显微内窥镜（HRME）在离体正常和恶性肝脏和胰腺组织中可视化细胞和结构特征的能力。评估超声医师使用HRME图像识别正常和肿瘤组织的能力。验证eus细针穿刺（FNP）在体HRME成像的初步技术可行性。离体，人体组织试点可行性研究；体内猪模型。两个学术医疗中心共同注册HRME图像和活检，从总共44名患者的手术切除的肝脏和胰腺组织中获得。图像被分为训练集（12张图像）和测试集（80张图像），其中包含每个器官的一系列正常和病理情况。在查看了训练集后，9名内窥镜技师试图在测试集中区分恶性组织与正常或良性病变，每个测试集包含40个独特的图像，每个图像都有来自病理学的单独诊断。图像采集的可行性，离体和体内。超声识别肝脏和胰腺正常/良性或恶性组织特征的能力。总体而言，9位超声仪在肝脏和胰腺的中位准确率分别为85%和90%。具有先前阅读HRME图像经验的超声医师的准确率在90%到95%之间。在猪体内模型中验证了通过19号EUS-FNP针进行HRME成像的技术可行性。离体研究高分辨率显微内窥镜可以实时成像肝脏和胰腺的细胞水平形态和组织结构。该技术似乎有一个短的学习曲线，在此之后，超声在区分两个器官的恶性组织与正常和良性病理方面取得了很高的准确率。将这种成像平台转化为体内环境在技术上是可行的。
Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is one of the few techniques which can obtain cells and tissue from the liver and pancreas. However, the technique remains vulnerable to poor specimen quality and sampling error. To evaluate the ability of a high-resolution microendoscope (HRME) to visualize the cellular and architectural features of normal and malignant liver and pancreatic tissue ex vivo. To assess the ability of endosonographers to identify normal and neoplastic tissue using HRME images. To demonstrate preliminary technical feasibility of in vivo HRME imaging via EUS-fine needle puncture (FNP). Ex vivo, pilot feasibility study in human tissue; in vivo swine model. Two academic medical centers Co-registered HRME images and biopsies were obtained from surgically resected hepatic and pancreatic tissues from a total of 44 patients. Images were divided into training (12 images) and test sets (80 images) containing a range of normal and pathologic conditions for each organ. After viewing the training sets, nine endosonographers attempted to distinguish malignant tissue from normal or benign lesions in the test sets, each of which contained 40 unique images with individual diagnoses from pathology. Image acquisition feasibility, ex vivo and in vivo. Ability of endosonographers to recognize features of normal/benign or malignant tissue from the liver and pancreas. Overall, the nine endosonographers achieved median accuracy figures of 85% in the liver and 90% in the pancreas. The endosonographers with prior experience in reading HRME images achieved accuracy rates between 90% and 95%. Technical feasibility of HRME imaging through a 19-gauge EUS-FNP needle was demonstrated in an in vivo swine model. Ex vivo study High-resolution microendoscopy allows real-time imaging of cellular-level morphology and tissue architecture in the liver and pancreas. The techniques appears to have a short learning curve, after which endosonographers achieved high accuracy rates in distinguishing malignant tissue from normal and benign pathology in both organs. Translating this imaging platform to the in vivo setting appears technically feasible.
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影响因子：
7.7
作者：
Suter, Melissa J.;Vakoc, Benjamin J.;Yachimski, Patrick S.;Shishkov, Milen;Lauwers, Gregory Y.;Mino-Kenudson, Mari;Bouma, Brett E.;Nishioka, Norman S.;Tearney, Guillermo J.
通讯作者：
Tearney, Guillermo J.
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
7.7
作者：
Becker, Valentin;Wallace, Michael B.;Meining, Alexander
通讯作者：
Meining, Alexander
影响因子：
7.7
作者：
Buchner, Anna M.;Gomez, Victoria;Wallace, Michael B.
通讯作者：
Wallace, Michael B.
影响因子：
24.5
作者：
Voss, M;Hammel, P;Ruszniewski, P
通讯作者：
Ruszniewski, P