Detection of a new bat gammaherpesvirus in the Philippines.
Detection of a new bat gammaherpesvirus in the Philippines.
复制标题
检测菲律宾的新蝙蝠伽马犬病毒。
DOI：
10.1007/s11262-009-0368-8
复制
发表时间：
2009-08
期刊：
影响因子：
1.6
通讯作者：
Akashi H
中科院分区：
文献类型：
--
作者：
Watanabe S;Ueda N;Iha K;Masangkay JS;Fujii H;Alviola P;Mizutani T;Maeda K;Yamane D;Walid A;Kato K;Kyuwa S;Tohya Y;Yoshikawa Y;Akashi H
在菲律宾班乃岛采集的一只食虫蝙蝠（蹄蝠科，蹄蝠属迪亚德马）的脾脏中检测到一种新的蝙蝠疱疹病毒。使用靶向疱疹病毒DNA聚合酶（DPOL）基因的CONsensus-DEgenerate杂交寡核苷酸引物（CODEHOPs）进行PCR分析。虽然我们用CODEHOPs获得了PCR产物，但由于高度简并性，使用引物直接测序是不可能的。本研究应用自行开发的病毒RNA序列快速测定系统（RDV）中的直接测序技术，测定了部分DPOL核苷酸序列。此外，部分gB基因的核苷酸序列也确定使用相同的策略。将gB基因和DPOL基因的部分序列进行长距离PCR连接，得到一个3741 bp的片段，包括gB基因的3′端和DPOL基因的5′端。系统发育分析表明，该序列是新的和最相似的亚科伽玛疱疹病毒。
A new bat herpesvirus was detected in the spleen of an insectivorous bat (Hipposideros diadema, family Hipposideridae) collected on Panay Island, the Philippines. PCR analyses were performed using COnsensus-DEgenerate Hybrid Oligonucleotide Primers (CODEHOPs) targeting the herpesvirus DNA polymerase (DPOL) gene. Although we obtained PCR products with CODEHOPs, direct sequencing using the primers was not possible because of high degree of degeneracy. Direct sequencing technology developed in our rapid determination system of viral RNA sequences (RDV) was applied in this study, and a partial DPOL nucleotide sequence was determined. In addition, a partial gB gene nucleotide sequence was also determined using the same strategy. We connected the partial gB and DPOL sequences with long-distance PCR, and a 3741-bp nucleotide fragment, including the 3′ part of the gB gene and the 5′ part of the DPOL gene, was finally determined. Phylogenetic analysis showed that the sequence was novel and most similar to those of the subfamily Gammaherpesvirinae.
登录
查看更多内容
影响因子：
11.8
作者：
Mizutani, Tetsuya;Endoh, Daiji;Nishimura, Hidekazu
通讯作者：
Nishimura, Hidekazu
DOI：
10.1016/j.cimid.2008.07.008
发表时间：
2010-01
期刊：
Comparative immunology, microbiology and infectious diseases
影响因子：
--
作者：
Watanabe S;Omatsu T;Miranda ME;Masangkay JS;Ueda N;Endo M;Kato K;Tohya Y;Yoshikawa Y;Akashi H
通讯作者：
Akashi H
影响因子：
10.7
作者：
Tamura, Koichiro;Dudley, Joel;Kumar, Sudhir
通讯作者：
Kumar, Sudhir
影响因子：
3.8
作者：
Wibbelt, Gudrun;Kurth, Andreas;Ehlers, Bernhard
通讯作者：
Ehlers, Bernhard
影响因子：
9.4
作者：
VanDevanter, DR;Warrener, P;Rose, TM
通讯作者：
Rose, TM