Epizootology and experimental infection of Yokose virus in bats.
Epizootology and experimental infection of Yokose virus in bats.
复制标题
DOI：
10.1016/j.cimid.2008.07.008
复制
发表时间：
2010-01
期刊：
影响因子：
--
通讯作者：
Akashi H
中科院分区：
文献类型：
--
作者：
Watanabe S;Omatsu T;Miranda ME;Masangkay JS;Ueda N;Endo M;Kato K;Tohya Y;Yoshikawa Y;Akashi H
为了揭示蝙蝠是否是YOKV(YOKV)的放大宿主，我们进行了一项血清学调查，并用从日本小蝙蝠身上分离的YOKV感染果蝠。YOKV属于黄病毒科黄病毒属中媒介未知组的恩德培蝙蝠病毒群。为了检测YOKV抗体，我们建立了用生物素标记的抗BAT-Ig G兔血清检测YOKV抗体的酶联免疫吸附试验(ELISA)。对在菲律宾采集的样本和从马来西亚提供的血清进行了血清学监测。来自菲律宾的36个样本中有1个(2.7%)和马来西亚的26个样本中有5个(19%)具有可检测到的ELISA抗体。在实验感染中，没有观察到疾病的临床症状。此外，没有检测到显著的病毒基因组扩增。这些发现揭示了YOKV在果蝠体内的复制能力很差，这表明果蝠似乎并不是YOKV的放大宿主。
To reveal whether bats serve as an amplifying host for Yokose virus (YOKV), we conducted a serological survey and experimentally infected fruit bats with YOKV isolated from microbats in Japan. YOKV belongs to the Entebbe bat virus group of vector unknown group within the genus Flavivirus and family Flaviviridae. To detect antibodies against YOKV, we developed an enzyme-linked immunosorbent assay (ELISA) using biotinylated anti-bat IgG rabbit sera. Serological surveillance was conducted with samples collected in the Philippines and the sera supplied from Malaysia. One of the 36 samples from the Philippines (2.7%) and 5 of the 26 samples from Malaysia (19%) had detectable ELISA antibodies. In the experimental infections, no clinical signs of disease were observed. Moreover, no significant viral genome amplification was detected. These findings revealed that YOKV replicates poorly in the fruit bat, suggesting that fruit bats do not seem to serve as an amplifying host for YOKV.
登录
查看更多内容
影响因子：
3.8
作者：
Billoir, F;de Chesse, R;de Lamballerie, X
通讯作者：
de Lamballerie, X
影响因子：
11.8
作者：
Arguin, PM;Murray-Lillibridge, K;Rupprecht, CE
通讯作者：
Rupprecht, CE
DOI：
10.4269/ajtmh.2006.75.1165
发表时间：
2006-12-01
影响因子：
3.3
作者：
Kuno, Goro;Chang, Gwong-Jen J.
通讯作者：
Chang, Gwong-Jen J.
影响因子：
3.8
作者：
Gaunt, MW;Sall, AA;Gould, EA
通讯作者：
Gould, EA
影响因子：
3.8
作者：
Charlier, N;Leyssen, P;Neyts, J
通讯作者：
Neyts, J