Characterization of excitatory and inhibitory neuron activation in the mouse medial prefrontal cortex following palatable food ingestion and food driven exploratory behavior.
Characterization of excitatory and inhibitory neuron activation in the mouse medial prefrontal cortex following palatable food ingestion and food driven exploratory behavior.
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DOI：
10.3389/fnana.2014.00060
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发表时间：
2014
影响因子：
2.9
通讯作者：
Scott MM
中科院分区：
文献类型：
--
作者：
Gaykema RP;Nguyen XM;Boehret JM;Lambeth PS;Joy-Gaba J;Warthen DM;Scott MM
内侧前额叶皮层 (mPFC) 与执行功能的各个方面有关，包括目标选择中涉及的注意力和记忆过程的调节。寻求食物的行为已被证明涉及 mPFC 的激活，无论是在执行旨在获取食物的策略期间还是在消耗食物本身期间。由于这些行为可能需要前额叶皮层的不同参与，我们假设神经元激活的模式也将依赖于行为。在这项研究中，我们首次描述了在食用可口食物和动物旨在获得食物奖励的探索活动后，小鼠 mPFC 的边缘下/背脚和前边缘/前扣带回细分的不同层和细胞类型中 Fos 的表达。虽然这两种操作都导致主要兴奋性神经元中 Fos 表达相对于对照增加，但以食物为导向的探索活动产生的 Fos 表达增加明显大于在食物摄入条件下观察到的增加。因此，我们假设 mPFC 中间神经元的激活也会受到这些操作的影响。有趣的是，Fos 表达模式在治疗和中间神经元亚型之间存在显着差异，说明 mPFC 中间神经元子集的差异参与如何取决于行为状态。在我们的实验中，表达血管活性肠肽和小白蛋白的神经元仅在食物依赖性探索任务期间而不是在食物摄入期间表现出 Fos 表达增强。相反，弓状和室旁下丘脑 fos 表达的升高仅在食物摄入后观察到，而不是在食物驱动的探索后观察到。我们的数据表明，可能需要选择性激活这些细胞类型来支持高认知需求状态，例如在探索过程中观察到的情况，而在摄入免费食物期间却是可有可无的。
The medial prefrontal cortex (mPFC) is implicated in aspects of executive function, that include the modulation of attentional and memory processes involved in goal selection. Food-seeking behavior has been shown to involve activation of the mPFC, both during the execution of strategies designed to obtain food and during the consumption of food itself. As these behaviors likely require differential engagement of the prefrontal cortex, we hypothesized that the pattern of neuronal activation would also be behavior dependent. In this study we describe, for the first time, the expression of Fos in different layers and cell types of the infralimbic/dorsal peduncular and prelimbic/anterior cingulate subdivisions of mouse mPFC following both the consumption of palatable food and following exploratory activity of the animal directed at obtaining food reward. While both manipulations led to increases of Fos expression in principal excitatory neurons relative to control, food-directed exploratory activity produced a significantly greater increase in Fos expression than observed in the food intake condition. Consequently, we hypothesized that mPFC interneuron activation would also be differentially engaged by these manipulations. Interestingly, Fos expression patterns differed substantially between treatments and interneuron subtype, illustrating how the differential engagement of subsets of mPFC interneurons depends on the behavioral state. In our experiments, both vasoactive intestinal peptide- and parvalbumin-expressing neurons showed enhanced Fos expression only during the food-dependent exploratory task and not during food intake. Conversely, elevations in arcuate and paraventricular hypothalamic fos expression were only observed following food intake and not following food driven exploration. Our data suggest that select activation of these cell types may be required to support high cognitive demand states such as observed during exploration while being dispensable during the ingestion of freely available food.
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