Antigenic and genetic characterization of influenza viruses isolated in Mozambique during the 2015 season.
Antigenic and genetic characterization of influenza viruses isolated in Mozambique during the 2015 season.
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2015季节，莫桑比克分离的流感病毒的抗原和遗传表征。
DOI：
10.1371/journal.pone.0201248
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
McCauley J
中科院分区：
文献类型：
--
作者：
Tivane A;Daniels R;Nguenha N;Machalele L;Nacoto A;Pale M;Mateonane E;Mavale S;Chilundo J;Muteto D;Salência J;Albati F;Gudo E;Mussá T;McCauley J
由于流感病毒的抗原变异率很高，病毒的季节性特征对于评估和监测新的致病变异的出现，从而制定有效的控制措施至关重要。然而，在莫桑比克尚未开展任何研究来评估流感病毒的遗传、抗原和抗病毒易感性谱。选取马普托市两家医院在2015年流感季节作为流感监测系统实施的一部分检测到的流感阳性儿童样本子集（n = 20）。对这些样本进行了以下检测：血凝抑制法进行抗原鉴定，血凝素（HA）和神经氨酸酶（NA）的Sanger测序进行遗传鉴定，酶促法对奥司他韦和扎那米韦（NA抑制剂）的敏感性。A(H1N1)pdm09亚型病毒与2016年疫苗病毒A/California/7/2009和其他广泛分布的属于遗传组6B的病毒在抗原性和遗传上密切相关。研究的大多数甲型流感（H3N2）病毒与2016-2017年疫苗病毒A/Hong Kong/4801/2014抗原相似，HA和NA基因序列均属于遗传亚支系3C。与非洲南部流行的病毒密切相关。B型流感病毒的抗原性与2016年季节疫苗病毒相似，HA序列均属于B/ yamagata谱系第3支，但含有B/ victoria谱系的NA基因。所有测试的病毒对奥司他韦和扎那米韦敏感。总体而言，所有莫桑比克甲型和乙型流感病毒与南部非洲病毒最密切相关，并且都对奥司他韦和扎那米韦敏感。这些发现表明，该地区存在流感病毒生态位，因此强调需要进行流行病学和病毒学联合监测，以监测流感病毒的演变。
Due to the high rate of antigenic variation of influenza virus, seasonal characterization of the virus is crucial to assess and monitor the emergence of new pathogenic variants and hence formulate effective control measures. However, no study has yet been conducted in Mozambique to assess genetic, antigenic and antiviral susceptibility profile of influenza virus. A subset of samples (n = 20) from influenza positive children detected in two hospitals in Maputo city during 2015 season as part of the implementation of influenza surveillance system, were selected. The following assays were performed on these samples: antigenic characterization by hemagglutination inhibition assay, genetic characterization by Sanger sequencing of hemagglutinin (HA) and neuraminidase (NA) and susceptibility to oseltamivir and zanamivir (NA inhibitors) by enzymatic assay. The A(H1N1)pdm09 subtype viruses remained closely related antigenically and genetically to the 2016 vaccine virus A/California/7/2009 and other widely distributed viruses belonging to genetic group 6B. The majority of influenza A(H3N2) viruses studied were antigenically similar to the 2016–2017 vaccine virus, A/Hong Kong/4801/2014, and their HA and NA gene sequences fell into genetic subclade 3C.2a being closely related to viruses circulating in southern Africa. The influenza B viruses were antigenically similar to the 2016 season vaccine virus and HA sequences of all three fell into the B/Yamagata-lineage, clade 3, but contained NA genes of the B/Victoria-lineage. All tested viruses were sensitive to oseltamivir and zanamivir. Overall, all Mozambican influenza A and B viruses were most closely related to Southern African viruses and all were sensitive to oseltamivir and zanamivir. These findings suggest the existence of an ecological niche of influenza viruses within the region and hence highlighting the need for joint epidemiologic and virologic surveillance to monitor the evolution of influenza viruses.
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影响因子：
2.9
作者：
POTIER, M;MAMELI, L;MELANCON, SB
通讯作者：
MELANCON, SB
DOI：
10.1016/j.ejpb.2015.05.023
发表时间：
2015-08-01
影响因子：
4.9
作者：
Soema, Peter C.;Kompier, Ronald;Kersten, Gideon F. A.
通讯作者：
Kersten, Gideon F. A.
影响因子：
3.7
作者：
ROBERTSON, JS;BOOTMAN, JS;SCHILD, GC
通讯作者：
SCHILD, GC
影响因子：
3.7
作者：
ROBERTSON, JS;NAEVE, CW;SCHILD, GC
通讯作者：
SCHILD, GC
影响因子：
4.4
作者：
Lin Y;Wharton SA;Whittaker L;Dai M;Ermetal B;Lo J;Pontoriero A;Baumeister E;Daniels RS;McCauley JW
通讯作者：
McCauley JW