Inactivation combined with cell lysis of Pseudomonas putida using a low pressure carbon dioxide microbubble technology.
Inactivation combined with cell lysis of Pseudomonas putida using a low pressure carbon dioxide microbubble technology.
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DOI：
10.1002/jctb.5299
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发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Zimmerman W
中科院分区：
文献类型：
--
作者：
Mulakhudair AR;Al-Mashhadani M;Hanotu J;Zimmerman W
灭活工艺可分为非热灭活方法（如环氧乙烷和γ辐射）和热方法（如高压灭菌）。在本研究中，研究了富含二氧化碳的微泡作为非热灭菌方法杀灭悬浮在生理盐水中的恶臭假单胞菌的能力，与传统的热灭菌和非热灭菌方法相比，该方法具有许多操作优势。引入富含二氧化碳的微泡可以在处理90分钟后实现细菌种群的102-Log减少，向灭活溶液中添加乙醇进一步增强灭活过程，以分别实现2%、5%和10%（v/v）乙醇的3、2.5和3.5-Log减少。在每次处理后，假单胞菌细胞上观察到一系列形态学变化，这些变化从细胞形状从杆状到球菌状的改变延伸到严重的病变和细胞死亡。恶臭假单胞菌KT 2440用作革兰氏阴性菌的模型。富CO2微泡技术具有能耗低（无热源）、避免使用有毒和腐蚀性试剂、可原位处理等优点。此外，这项研究的许多发现也适用于其他革兰氏阴性菌。版权所有© 2017作者. Journal of Chemical Technology & Biotechnology由John Wiley & Sons Ltd代表化学工业协会出版。
Inactivation processes can be classified into non‐thermal inactivation methods such as ethylene oxide and γ‐radiation, and thermal methods such as autoclaving. The ability of carbon dioxide enriched microbubbles to inactivate Pseudomonas putida suspended in physiological saline, as a non‐thermal sterilisation method, was investigated in this study with many operational advantages over both traditional thermal and non‐thermal sterilisation methods. Introducing carbon dioxide enriched microbubbles can achieve ∼2‐Log reduction in the bacterial population after 90 min of treatment, addition of ethanol to the inactivation solution further enhanced the inactivation process to achieve 3, 2.5 and 3.5‐Log reduction for 2%, 5% and 10 %( v/v) ethanol, respectively. A range of morphological changes was observed on Pseudomonas cells after each treatment, and these changes extended from changing cell shape from rod shape to coccus shape to severe lesions and cell death. Pseudomonas putida KT 2440 was used as a model of gram‐negative bacteria. Using CO2 enriched microbubbles technology has many advantages such as efficient energy consumption (no heat source), avoidance of toxic and corrosive reagents, and in situ treatment. In addition, many findings from this study could apply to other gram‐negative bacteria. © 2017 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
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影响因子：
4.4
作者：
Heipieper, HJ;Meulenbeld, G;DeBont, JAM
通讯作者：
DeBont, JAM
影响因子：
4.4
作者：
HEIPIEPER, HJ;KEWELOH, H;REHM, HJ
通讯作者：
REHM, HJ
影响因子：
17.3
作者：
HEIPIEPER, HJ;WEBER, FJ;DEBONT, JAM
通讯作者：
DEBONT, JAM
影响因子：
5.1
作者：
Hallsworth, JE;Heim, S;Timmis, KN
通讯作者：
Timmis, KN
影响因子：
4.3
作者：
CARROLL, JJ;SLUPSKY, JD;MATHER, AE
通讯作者：
MATHER, AE