Bioallethrin activates specific olfactory sensory neurons and elicits spatial repellency in Aedes aegypti.
Bioallethrin activates specific olfactory sensory neurons and elicits spatial repellency in Aedes aegypti.
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DOI：
10.1002/ps.6682
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发表时间：
2022-03
影响因子：
4.1
通讯作者：
Dong K
中科院分区：
文献类型：
--
作者：
Valbon W;Andreazza F;Oliveira EE;Liu F;Feng B;Hall M;Klimavicz J;Coats JR;Dong K
使用拟除虫菊酯类杀虫剂是全球蚊虫控制的关键战略。挥发性拟除虫菊酯，如生物丙烯菊酯和反氟菊酯，通常因其作用于电压门控钠通道的杀虫活性而闻名，用于蚊香、喷雾器和其他汽化器，以驱蚊和其他咬人的节肢动物。然而，是否特定的嗅觉受体神经元被拟除虫菊酯激活以触发空间排斥仍然未知。采用行为学和电生理学方法研究了登革热、黄热病、寨卡病毒和基孔肯雅病毒的主要传播媒介埃及伊蚊对丙烯菊酯的驱避机制。我们发现，生物丙烯菊酯引起空间（即非接触）驱避，并激活蚊子触角中的一种特定类型的嗅觉受体神经元。此外，生物丙烯菊酯的驱蚊性在蚊子的Orco突变体中显著降低，Orco是一种专性嗅觉共受体，对气味受体（Ors）的功能至关重要。这些结果表明，生物丙烯菊酯激活特定的Or(s)有助于生物丙烯菊酯驱避。此外，在一个具有拟除虫菊酯抗性的菌株中，钠通道基因的两个突变导致了对拟除虫菊酯的敲除抗性（kdr），表明钠通道的激活在生物丙烯菊酯的驱避中起作用。本研究结果表明，生物丙烯菊酯的驱避作用可能是Or(s)和钠离子通道共同激活的结果。这些发现不仅有助于了解挥发性拟除虫菊酯在空间驱避中的作用模式，而且为基于所揭示的双靶点机制开发新型驱避剂提供了框架。
Use of pyrethroid insecticides is a pivotal strategy for mosquito control globally. Commonly known for its insecticidal activity by acting on voltage-gated sodium channels, volatile pyrethroids, such as bioallethrin and transfluthrin, are used in mosquito coils, emanators, and other vaporizers to repel mosquitoes and other biting arthropods. However, whether specific olfactory receptor neurons are activated by pyrethroids to trigger spatial repellency remains unknown. We took behavioral and electrophysiological approaches to elucidate the mechanism of bioallethrin repellency in Aedes aegypti, a major vector of dengue, yellow fever, Zika and chikungunya viruses. We found that bioallethrin elicits spatial (i.e., non-contact) repellency and activates a specific type of olfactory receptor neurons in mosquito antennae. Furthermore, bioallethrin repellency is significantly reduced in a mosquito mutant of Orco, an obligate olfactory co-receptor that is essential for the function of odorant receptors (Ors). These results indicate that activation of specific Or(s) by bioallethrin contributes to bioallethrin repellency. In addition, bioallethrin repellency was reduced in a pyrethroid-resistant strain which carries two mutations in the sodium channel gene that are responsible for knockdown resistance (kdr) to pyrethroids, indicating a role of activation of sodium channels in bioallethrin repellency. Results from this study show that bioallethrin repellency is likely the result of co-activation of Or(s) and sodium channels. These findings not only contribute to the understanding of the modes of action of volatile pyrethroids in spatial repellency, but also provide a framework for developing new repellents based on the dual-target mechanism revealed.
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DOI：
10.1111/j.1460-9568.2007.05786.x
发表时间：
2007-09
期刊：
The European journal of neuroscience
影响因子：
--
作者：
Ghaninia M;Ignell R;Hansson BS
通讯作者：
Hansson BS
影响因子：
2.2
作者：
Chin, A. C.;Chen, C. D.;Sofian-Azirun, M.
通讯作者：
Sofian-Azirun, M.
影响因子：
3.8
作者：
Hirata K;Komagata O;Itokawa K;Yamamoto A;Tomita T;Kasai S
通讯作者：
Kasai S
影响因子：
3.8
作者：
Kasai S;Komagata O;Itokawa K;Shono T;Ng LC;Kobayashi M;Tomita T
通讯作者：
Tomita T
影响因子：
16.2
作者：
Larsson, MC;Domingos, AI;Vosshall, LB
通讯作者：
Vosshall, LB