A single crossing-over event in voltage-sensitive Na+ channel genes may cause critical failure of dengue mosquito control by insecticides.
A single crossing-over event in voltage-sensitive Na+ channel genes may cause critical failure of dengue mosquito control by insecticides.
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DOI：
10.1371/journal.pntd.0003085
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发表时间：
2014-08
影响因子：
3.8
通讯作者：
Kasai S
中科院分区：
文献类型：
--
作者：
Hirata K;Komagata O;Itokawa K;Yamamoto A;Tomita T;Kasai S
电压敏感性钠（Na+）通道（Vssc）是拟除虫菊酯类杀虫剂的靶位点。害虫通过在该通道中获得一个或多个氨基酸取代而对这类杀虫剂产生抗性。在东南亚，两种主要的Vssc类型赋予登革热蚊子媒介埃及伊蚊（Aedes aegypti）对拟除虫菊酯的抗性，即S989 P + V1016 G和F1534 C。我们在非洲爪蟾卵母细胞中表达了几种类型的Vssc，并研究了Vssc中氨基酸取代对拟除虫菊酯敏感性的影响。S989 P + V1016 G和F1534 C单倍型分别使通道对氯菊酯的敏感性降低100倍和25倍，而S989 P + V1016 G + F1534 C三重突变使通道对氯菊酯的敏感性降低1100倍。S989 P + V1016 G和F1534 C单倍型分别使通道对溴氰菊酯的敏感性降低10倍和1倍（无降低），但S989 P + V1016 G + F1534 C三重突变使通道对溴氰菊酯的敏感性降低90倍。这些结果表明，拟除虫菊酯杀虫剂很可能失去对A。如果这样的Vssc单倍型作为单一交换事件的结果出现，则可能是埃及伊蚊;因此，这可能导致控制这种关键蚊子媒介的失败。在此，我们强调了在田间种群中监测Vssc三重突变的重要性。埃及人。拟除虫菊酯是广泛用于控制蚊媒的主要类别的杀虫剂之一。拟除虫菊酯的靶位点位于由约2100个氨基酸残基组成的电压敏感性Na+通道（Vssc）中。在这项研究中，我们产生了几种类型的Vssc与一个或多个突变，表达在非洲爪蟾卵母细胞，并研究其电生理特性，使用双电极电压钳方法。我们证实，携带三重突变的埃及伊蚊Vssc对拟除虫菊酯类杀虫剂表现出极高的抗性水平。这种Vssc类型可以通过广泛分布在东南亚（最大的登革热流行地区之一）的两个抗性Vssc基因的单一交叉事件产生。我们的研究结果强调了加强监测A.埃及蚊
The voltage-sensitive sodium (Na+) channel (Vssc) is the target site of pyrethroid insecticides. Pest insects develop resistance to this class of insecticide by acquisition of one or multiple amino acid substitution(s) in this channel. In Southeast Asia, two major Vssc types confer pyrethroid resistance in the dengue mosquito vector Aedes aegypti, namely, S989P+V1016G and F1534C. We expressed several types of Vssc in Xenopus oocytes and examined the effect of amino acid substitutions in Vssc on pyrethroid susceptibilities. S989P+V1016G and F1534C haplotypes reduced the channel sensitivity to permethrin by 100- and 25-fold, respectively, while S989P+V1016G+F1534C triple mutations reduced the channel sensitivity to permethrin by 1100-fold. S989P+V1016G and F1534C haplotypes reduced the channel sensitivity to deltamethrin by 10- and 1-fold (no reduction), respectively, but S989P+V1016G+F1534C triple mutations reduced the channel sensitivity to deltamethrin by 90-fold. These results imply that pyrethroid insecticides are highly likely to lose their effectiveness against A. aegypti if such a Vssc haplotype emerges as the result of a single crossing-over event; thus, this may cause failure to control this key mosquito vector. Here, we strongly emphasize the importance of monitoring the occurrence of triple mutations in Vssc in the field population of A. aegypti. Pyrethroids are one of the major classes of insecticides that is widely used to control mosquito vectors. The target site of pyrethroids is found in the voltage-sensitive Na+ channel (Vssc) consisting of about 2100 amino acid residues. In this study we generated several types of Vssc with a single or multiple mutations, expressed in Xenopus oocytes, and examined their electrophysiological properties using two-electrode voltage clamp method. We confirmed that Aedes aegypti Vssc harboring a triple mutations exhibited extremely high levels of resistance to pyrethroid insecticides. This Vssc type can be generated by a single crossing-over event of two resistant Vssc genes that are widely distributed in Southeast Asia, one of the greatest dengue endemic areas. Our results highlight the importance of intensive monitoring for the triple mutations in Vssc in the A. aegypti mosquito.
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影响因子：
3.8
作者：
Hu, Zhaonong;Du, Yuzhe;Nomura, Yoshiko;Dong, Ke
通讯作者：
Dong, Ke
影响因子：
3.8
作者：
Burton, Mark J.;Mellor, Ian R.;Williamson, Martin S.
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影响因子：
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作者：
Kawada H;Higa Y;Komagata O;Kasai S;Tomita T;Thi Yen N;Loan LL;Sánchez RA;Takagi M
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影响因子：
3.8
作者：
Kasai S;Komagata O;Itokawa K;Shono T;Ng LC;Kobayashi M;Tomita T
通讯作者：
Tomita T
影响因子：
2.2
作者：
Amdam, GV;Hartfelder, K;Omholt, SW
通讯作者：
Omholt, SW