The genome segment B encoding the RNA-dependent RNA polymerase protein VP1 of very virulent infectious bursal disease virus (IBDV) is phylogenetically distinct from that of all other IBDV strains.
The genome segment B encoding the RNA-dependent RNA polymerase protein VP1 of very virulent infectious bursal disease virus (IBDV) is phylogenetically distinct from that of all other IBDV strains.
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DOI：
10.1007/s007050170018
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发表时间：
2001-12
影响因子：
2.7
通讯作者：
Müller H
中科院分区：
文献类型：
--
作者：
Islam MR;Zierenberg K;Müller H
构建了传染性法氏囊病病毒（IBDV）强毒株BD 3/99株B片段的全长cDNA克隆，并测定了其核苷酸序列。将BD 3/99株与其它17株IBDV毒株（包括6株超强毒株、3株经典强毒株、5株经典弱毒株、1株抗原变异株和2株血清2型毒株）的VP 1基因序列进行了同源性比较。所有强毒株的VP 1基因同源性为97.5%~ 99.8%。除了非典型的澳大利亚菌株002-73外，所有经典的强毒株或减毒株和抗原变异株也具有97.5%至100%的相同性。血清2型毒株与血清1型经典强毒株或减毒株的差异仅为4-6%，而与之相反，超强毒株与经典强毒株或减毒株以及血清2型毒株的差异为10.5%-12.5%。VP 1氨基酸序列分析显示，在强毒株中有17个共同的氨基酸替换，其中8个是独特的。在系统发育树中，极强毒株形成了一个独特的簇，所有其他毒株，包括经典的强毒株、减毒株和抗原变异株，甚至血清2型毒株都聚在一起。这表明，超强毒株的VP 1基因在遗传学上与其它毒株不同，可能来源于一个迄今尚未确定的来源。
A full-length cDNA clone of the segment B of the very virulent infectious bursal disease virus (IBDV) strain BD 3/99 was constructed and the full-length nucleotide sequence was established. The nucleotide sequence encoding VP1, an RNA-dependent RNA polymerase, of BD 3/99 was aligned with that of 17 other IBDV strains including six very virulent, three classical virulent, five classical attenuated, one antigenic variant and two serotype 2 strains. The VP1 genes of all very virulent strains were 97.5% to 99.8% identical. With the exception of an atypical Australian strain, 002-73, all of the classical virulent or attenuated and antigenic variant strains were also 97.5% to 100% identical. Serotype 2 strains showed only 4–6% divergence from serotype 1 classical virulent or attenuated strains; in contrast, however, the very virulent strains were 10.5% to 12.5% divergent from the classical virulent or attenuated strains as well as serotype 2 strains. Analysis of the deduced amino acid sequence of VP1 revealed 17 common, including 8 unique amino acid substitutions in the very virulent strains. In the phylogenetic tree the very virulent strains formed a distinct cluster and all other strains including classical virulent, attenuated and antigenic variant strains and even serotype 2 strains were grouped together. It to is suggested that the VP1 of very virulent IBDV is phylogenetically distinct from that of all other IBDV strains and probably originated from a hitherto unidentified source.
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影响因子：
3.8
作者：
BROWN, MD;GREEN, P;SKINNER, MA
通讯作者：
SKINNER, MA
影响因子：
3.7
作者：
Duncan R;Mason CL;Nagy E;Leong JA;Dobos P
通讯作者：
Dobos P
影响因子：
3.7
作者：
AZAD, AA;BARRETT, SA;FAHEY, KJ
通讯作者：
FAHEY, KJ
影响因子：
2.7
作者：
Eterradossi, N;Arnauld, C;Rivallan, G
通讯作者：
Rivallan, G
影响因子：
5.4
作者：
Lohmann, V;Korner, F;Bartenschlager, R
通讯作者：
Bartenschlager, R