Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif.
Sequence analysis of infectious pancreatic necrosis virus genome segment B and its encoded VP1 protein: a putative RNA-dependent RNA polymerase lacking the Gly-Asp-Asp motif.
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DOI：
10.1016/0042-6822(91)90887-h
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发表时间：
1991-04
期刊：
影响因子：
3.7
通讯作者：
Dobos P
中科院分区：
文献类型：
--
作者：
Duncan R;Mason CL;Nagy E;Leong JA;Dobos P
确定了碧玉和Sp血清型的传染性胰腺坏死病毒的基因组片段B序列。这两个序列的长度分别为2784和2630，含有一个编码VP 1蛋白的大的开放阅读框，VP 1蛋白是IPNV的假定的RNA依赖性RNA聚合酶（RdRp）。这些蛋白彼此之间具有88%的同源性，但与传染性法氏囊病病毒（IBDV）VP 1（双RNA病毒科的另一个成员）的同源性仅为41%。尽管IPNV和IBDV VP 1蛋白之间的总体同源性低，但在蛋白的中心部分内检测到同源区域。VP 1的羧基近端区域含有非常低的氨基酸同源性，显示出结构特征保守的证据，例如亲水性、高碱性结构域。在VP 1中也检测到与GTP结合蛋白和RdRps相关的共有序列。然而，与单链加RNA病毒相关的RdRps不同，双RNA病毒RdRp缺乏该酶家族的Gly-Asp-Asp基序特征。
The genome segment B sequence of infectious pancreatic necrosis virus was determined for both the Jasper and Sp serotypes. The sequences are 2784 and 2630 by long, respectively, and contain a single large open reading frame encoding the VP1 protein, the putative RNA-dependent RNA polymerase (RdRp) of IPNV. The proteins exhibit an 88% homology with each other, but only 41% with infectious bursal disease virus (IBDV) VP1, another member of the Birnaviridae. Despite the low overall homology between the IPNV and IBDV VP1 proteins, homologous regions were detected within the central portion of the proteins. The carboxy-proximal regions of the VP1, which contain very low amino acid homology, displayed evidence of conservation in structural features such as a hydrophilic, highly basic domain. Consensus sequences associated with GTP-binding proteins and RdRps were also detected in VP1. However, unlike the RdRps associated with single-stranded plus RNA viruses, the birnavirus RdRp lacks the Gly-Asp-Asp motif characteristic of this enzyme family.
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影响因子：
14.9
作者：
Gorbalenya AE;Koonin EV;Donchenko AP;Blinov VM
通讯作者：
Blinov VM
影响因子：
64.5
作者：
FIELDS, S;WINTER, G
通讯作者：
WINTER, G
影响因子：
14.9
作者：
DEVEREUX, J;HAEBERLI, P;SMITHIES, O
通讯作者：
SMITHIES, O
DOI：
10.1111/j.1432-1033.1985.tb09244.x
发表时间：
1985-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
ARGOS, P;LEBERMAN, R
通讯作者：
LEBERMAN, R
影响因子：
3.7
作者：
AZAD, AA;BARRETT, SA;FAHEY, KJ
通讯作者：
FAHEY, KJ