Comparative transcriptomic analysis of larval and adult Malpighian tubules from the cotton bollworm Helicoverpa armigera
Comparative transcriptomic analysis of larval and adult Malpighian tubules from the cotton bollworm Helicoverpa armigera
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棉铃虫棉铃虫幼虫和成虫马氏小管的比较转录组分析
DOI：
10.1111/1744-7917.12561
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发表时间：
2018-12
期刊：
影响因子：
4
通讯作者：
Xing Hui Qiu
中科院分区：
文献类型：
--
作者：
Yi Yang Yuan;Mei Li;Fan Fan;Xing Hui Qiu
马氏管（Malpighian tubules，MT）是昆虫重要的排泄和排泄调节器官。然而，越来越多的证据表明，MTs执行的功能不仅仅是肌肉调节。棉铃虫是一种重要的农业害虫，其MTs的分子和生理功能至今仍不清楚。本研究对H.使用RNA-Seq技术对来自幼虫、雄性成虫和雌性成虫的棉铃虫MT进行测序，并对两个生命阶段（幼虫和成虫）之间以及成虫性别之间的转录组进行比较分析。我们共产生了84 643个高质量的unigenes，并鉴定了大量丰富的转录本puplatin编码的蛋白质参与利尿，解毒，免疫，碳水化合物运输和代谢，发育和繁殖。我们发现，unigenes的表达模式是相对相似的雌，雄成人的MT，但不同的幼虫和成人的MT。我们的数据表明，昆虫的MT可能采取多种生理功能作为多才多艺的器官。从幼虫到成虫发生的基因表达的广泛变化反映了对不同食性的生态适应。棉铃虫雌雄成虫MT中与碳水化合物代谢、解毒、免疫和生殖相关基因的转录差异在一定程度上表明了棉铃虫的性二型性。
Malpighian tubules (MTs) are usually considered the key excretory and osmoregulatory organs of insects. However, increasing evidence has suggested that MTs perform many more functions than just osmoregulation. Until now, the molecular and physiological functions of MTs in the cotton bollworm (Helicoverpa armigera), a very important agricultural pest, are largely unknown. In this study, the transcriptomes of H. armigera MTs from larvae, male adults and female adults were sequenced using RNA‐Seq technology, and comparative analyses of transcriptomes between two life stages (larval and adult) and between adult sexes were conducted. We generated a total of 84 643 high‐quality unigenes, and identified a large number of abundant transcripts putatively encoding proteins involved in diuresis, detoxification, immunity, carbohydrate transport and metabolism, development and reproduction. We found that the expression pattern of unigenes was relatively similar between female and male adult MTs, but different between larval and adult MTs. Our data suggest that insect MTs may take multiple physiological functions as versatile organs. The extensive alterations in gene expression in MTs occurred from larvae to adults reflect an ecological adaptation to different feeding habits. Sexual dimorphism in the cotton bollworm is somewhat indicated by the transcriptional difference of genes related to carbohydrate metabolism, detoxification, immunity and reproduction in the MTs of male and female adults.
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DOI：
10.4238/2015.january.23.21
发表时间：
2015-01
期刊：
Genetics and molecular research : GMR
影响因子：
--
作者：
J. R. Silva;R. Prado;D. Amaral;V. Viviani
通讯作者：
J. R. Silva;R. Prado;D. Amaral;V. Viviani
影响因子：
3.7
作者：
Marlin D;Nicolson SW;Yusuf AA;Stevenson PC;Heyman HM;Krüger K
通讯作者：
Krüger K
DOI：
10.1016/s0140-6736(80)91745-6
发表时间：
1980-06
期刊：
The Federal Theology of Johannes Cocceius (1603-1669)
影响因子：
--
作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
通讯作者：
George Vogiatzis;Carlos Hernández;R. Cipolla
DOI：
--
发表时间：
2002-07
期刊：
The Journal of experimental biology
影响因子：
--
作者：
N. Skaer;D. Nässel;S. Maddrell;N. Tublitz
通讯作者：
N. Skaer;D. Nässel;S. Maddrell;N. Tublitz
与棉铃虫发育和 2-十三酮耐受性相关的转铁蛋白基因
影响因子：
2.6
作者：
Zhang L;Shang Q;Lu Y;Zhao Q;Gao X
通讯作者：
Gao X