The ABA-induced NAC transcription factor MdNAC1 interacts with a bZIP-type transcription factor to promote anthocyanin synthesis in red-fleshed apples.

The ABA-induced NAC transcription factor MdNAC1 interacts with a bZIP-type transcription factor to promote anthocyanin synthesis in red-fleshed apples.
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DOI:
10.1093/hr/uhad049
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发表时间:
2023-05
影响因子:
8.7
通讯作者:
--
中科院分区:
农林科学1区
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--
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花青素是红肉苹果中的重要化合物。MdMYB10转录因子是花青素合成途径的重要调节因子。然而,其他转录因子是控制花青素苷合成的复杂网络的关键组成部分,应该更彻底地表征。在这项研究中,我们使用了基于酵母的筛选技术,以确定MdNAC1作为一个转录因子,积极调节花青素的合成。在苹果果实和愈伤组织中过量表达MdNAC1显著促进了花青苷的积累。在结合实验中,我们证明了MdNAC1与bZIP型转录因子MdbZIP 23结合激活MdMYB 10和MdUFGT的转录。我们的分析还表明,MdNAC1的表达强烈诱导阿坝,因为存在的ABRE顺式作用元件在其启动子。此外,阿坝的存在下,在苹果愈伤组织中的花青素的积累增加与MdNAC 1和MdbZIP 23共转化。因此,我们揭示了一种新的花青素合成机制涉及ABA诱导的转录因子MdNAC1在红肉苹果。
Anthocyanins are valuable compounds in red-fleshed apples. The MdMYB10 transcription factor is an important regulator of the anthocyanin synthesis pathway. However, other transcription factors are key components of the complex network controlling anthocyanin synthesis and should be more thoroughly characterized. In this study, we used a yeast-based screening technology to identify MdNAC1 as a transcription factor that positively regulates anthocyanin synthesis. The overexpression of MdNAC1 in apple fruits and calli significantly promoted the accumulation of anthocyanins. In binding experiments, we demonstrated that MdNAC1 combines with the bZIP-type transcription factor MdbZIP23 to activate the transcription of MdMYB10 and MdUFGT. Our analyses also indicated that the expression of MdNAC1 is strongly induced by ABA because of the presence of an ABRE cis-acting element in its promoter. Additionally, the accumulation of anthocyanins in apple calli co-transformed with MdNAC1 and MdbZIP23 increased in the presence of ABA. Therefore, we revealed a novel anthocyanin synthesis mechanism involving the ABA-induced transcription factor MdNAC1 in red-fleshed apples.
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