Effect of storage conditions on the assessment of bacterial community structure in soil and human-associated samples.
Effect of storage conditions on the assessment of bacterial community structure in soil and human-associated samples.
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DOI：
10.1111/j.1574-6968.2010.01965.x
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发表时间：
2010-06
影响因子：
2.1
通讯作者：
Fierer N
中科院分区：
文献类型：
--
作者：
Lauber CL;Zhou N;Gordon JI;Knight R;Fierer N
储存条件被认为是基于DNA的微生物群落分析方法的关键组成部分。然而，短期样品储存条件的差异是否会影响细菌群落组成和多样性的评估，需要系统和定量的评估。因此，我们使用细菌16S rRNA基因的条形码焦磷酸测序来调查社区，从各种栖息地（土壤，人类肠道（粪便）和人类皮肤）中收获，随后在20°，4°，−20°和−80°C下储存3天和14天。我们的研究结果表明，在个别样品的系统发育结构和多样性的影响不显着的存储温度或存储时间。同样，大多数类群的相对丰度在很大程度上不受温度的影响，即使在14天的存储。我们的研究结果表明，环境因素和分子技术的偏差可能会给微生物群落带来更大的变化，而不是短期储存条件的差异，包括在室温下储存长达两周。这些结果表明，许多样品收集和储存在现场条件下没有冷藏可能是有用的微生物群落分析。
Storage conditions are considered to be a critical component of DNA-based microbial community analysis methods. However, whether differences in short-term sample storage conditions impact the assessment of bacterial community composition and diversity demands systematic and quantitative assessment. Therefore, we used barcoded pyrosequencing of bacterial 16S rRNA genes to survey communities, harvested from a variety of habitats (soil, human gut (feces) and human skin) and subsequently stored at 20°, 4°, −20°, and −80°C for 3 and 14 days. Our results indicate that the phylogenetic structure and diversity of communities in individual samples was not significantly influenced by storage temperature or duration of storage. Likewise, the relative abundances of most taxa were largely unaffected by temperature even after 14 days of storage. Our results indicate that environmental factors and biases in molecular techniques likely impart greater amounts of variation to microbial communities than do differences in short-term storage conditions, including storage for up to two weeks at room temperature. These results suggest that many samples collected and stored under field conditions without refrigeration may be useful for microbial community analyses.
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影响因子：
10.7
作者：
Price MN;Dehal PS;Arkin AP
通讯作者：
Arkin AP
DOI：
10.2174/1874285800903010040
发表时间：
2009
期刊：
The open microbiology journal
影响因子：
--
作者：
Roesch LF;Casella G;Simell O;Krischer J;Wasserfall CH;Schatz D;Atkinson MA;Neu J;Triplett EW
通讯作者：
Triplett EW
DOI：
10.1073/pnas.0807920105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Fierer, Noah;Hamady, Micah;Knight, Rob
通讯作者：
Knight, Rob
影响因子：
4.4
作者：
Lauber, Christian L.;Hamady, Micah;Fierer, Noah
通讯作者：
Fierer, Noah
影响因子：
5.9
作者：
FAITH, DP
通讯作者：
FAITH, DP