Monitoring glycation using the intrinsic fluorescence of biological fluorophores
Monitoring glycation using the intrinsic fluorescence of biological fluorophores
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使用生物荧光团的固有荧光监测糖化
DOI：
10.1117/12.2631649
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Muir R
中科院分区：
文献类型：
--
作者：
Muir R
与糖尿病相关的高血糖水平会影响体内的各种细胞和蛋白质。为了应对高血糖，胶原蛋白和角蛋白会发生糖化。这项工作旨在确定胶原蛋白和角蛋白的固有荧光是否可用于监测所述化合物的糖化，从而提供监测长期血糖控制的替代方法。我们使用稳态和时间分辨荧光光谱技术研究了两种化合物在体外响应葡萄糖的固有荧光的演变。观察到胶原蛋白和角蛋白的内在荧光的变化。对于胶原蛋白，与任意选择的激发和检测波长下的传统荧光强度衰减测量相反，我们进行了系统的波长和时间分辨测量，以获得时间分辨发射光谱（TRES）。这些显示了由胶原蛋白聚集和糖化引起的内在荧光动力学的变化。在角蛋白中，由于新交联的形成速度更快，葡萄糖的添加导致 460 nm 特征波长处的荧光强度增加。结果还表明，葡萄糖可能导致两种新的荧光复合物的形成，其荧光峰值位于~525 nm 和~575 nm。总之，监测胶原蛋白或角蛋白的内在荧光可用作监测糖尿病患者长期血糖控制的方法。
The high blood glucose levels associated with diabetes affect various cells and proteins in the body. In response to high blood glucose collagen and keratin proteins experience glycation. This work aims to establish if the intrinsic fluorescence of collagen and keratin could be used to monitor the glycation of said compounds, and thus offer an alternative method to monitoring long term glycaemic control. We have studied the evolution of the intrinsic fluorescence of both compounds in response to glucose in vitro, using steady state and time-resolved fluorescence spectroscopy techniques. Changes in the intrinsic fluorescence of both collagen and keratin were observed. For collagen, contrary to the traditional fluorescence intensity decay measurement at arbitrarily selected excitation and detection wavelengths, we conducted systematic wavelength- and time-resolved measurements to achieve time-resolved emission spectra (TRES). These showed changes in the intrinsic fluorescence kinetics, caused by both collagen aggregation and glycation. In keratin, the addition of glucose caused an increase in the fluorescence intensity at the characteristic wavelength of 460 nm, due to faster formation of new cross-links. The results also suggest that glucose may cause the formation of two new fluorescent complexes with peak fluorescence at ~525 nm and ~575 nm. In conclusion, monitoring the intrinsic fluorescence of collagen or keratin could be used as a method to monitor long term glycaemic control in patients with diabetes.
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影响因子：
10.3
作者：
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影响因子：
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作者：
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DOI：
10.1136/bmj.288.6418.669
发表时间：
1984
期刊：
British Medical Journal (Clinical research ed.)
影响因子：
--
作者：
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通讯作者：
M. Hartog
影响因子：
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作者：
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Delanghe JR