Glycation of nail proteins: from basic biochemical findings to a representative marker for diabetic glycation-associated target organ damage.
Glycation of nail proteins: from basic biochemical findings to a representative marker for diabetic glycation-associated target organ damage.
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DOI：
10.1371/journal.pone.0120112
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Delanghe JR
中科院分区：
文献类型：
--
作者：
Kishabongo AS;Katchunga P;Van Aken EH;Speeckaert R;Lagniau S;Coopman R;Speeckaert MM;Delanghe JR
尽管糖化指甲蛋白的评估可能是监测糖尿病的有用标志物，但其性质和形成仍然知之甚少。除了角蛋白糖化的详细解剖分析，糖化甲蛋白评估监测糖尿病并发症的有用性进行了研究。入组了216例患者（94例男性，122例女性;平均年龄±标准差：75.0 ± 8.7岁）。甲和眼透镜蛋白的糖化使用基于氮蓝四唑的光度测定法进行评估。提取的指甲蛋白质，结合和非结合级分的色谱分离后，使用一维凝胶电泳进行分析。使用包含闩锁型钻头的手柄，将甲板细致地切割成浅表层和深层，然后进行果糖胺的差异分析。使用SDS PAGE，分别在非糖基化和糖基化指甲组分中鉴定出4条和2条带。甲浅层果糖胺浓度（平均值：2.16 ± 1.37 μmol/g甲）显著低于甲深层果糖胺浓度（平均值：4.36 ± 2.55 μmol/g甲）（P<0.05）。糖尿病患者眼透镜糖基化蛋白含量（平均3.80 ± 1.57 μmol/g眼透镜）显著高于非糖尿病患者（平均3.35 ± 1.34 μmol/g眼透镜）（P<0.05）。甲和眼透镜蛋白的糖基化程度有显著相关性[y（甲糖基化蛋白）= 0.39 + 0.99 x（眼透镜糖基化蛋白）; r2 = 0.58，P<0.001]。发现糖化眼透镜蛋白的浓度和HbA 1c水平是糖化指甲蛋白浓度的预测因子。指甲蛋白的糖化发生在指甲的深层，与血管和间质液密切接触。甲蛋白的糖基化可被视为糖尿病糖基化相关靶器官损害的代表性标志物。
Although assessment of glycated nail proteins may be a useful marker for monitoring of diabetes, their nature and formation are still poorly understood. Besides a detailed anatomical analysis of keratin glycation, the usefulness of glycated nail protein assessment for monitoring diabetic complications was investigated. 216 patients (94 males, 122 females; mean age ± standard deviation: 75.0 ± 8.7 years) were enrolled. Glycation of nail and eye lens proteins was assessed using a photometric nitroblue tetrazolium-based assay. Following chromatographic separation of extracted nail proteins, binding and nonbinding fractions were analyzed using one-dimensional gel electrophoresis. Using a hand piece containing a latch-type-bur, a meticulous cutting of the nail plate into superficial and deep layers was performed, followed by a differential analysis of fructosamine. Using SDS PAGE, four and two bands were identified among the nonglycated and glycated nail fraction respectively. Significantly lower fructosamine concentrations were found in the superficial nail layer (mean: 2.16 ± 1.37 μmol/g nails) in comparison with the deep layer (mean: 4.36 ± 2.55 μmol/g nails) (P<0.05). A significant higher amount of glycated eye lens proteins was found in diabetes mellitus patients (mean: 3.80 ± 1.57 μmol/g eye lens) in comparison with nondiabetics (mean: 3.35 ± 1.34 μmol/g eye lens) (P<0.05). A marked correlation was found between glycated nail and glycated eye lens proteins [y (glycated nail proteins) = 0.39 + 0.99 x (eye lens glycated proteins); r2 = 0.58, P<0.001]. The concentration of glycated eye lens proteins and the HbA1c level were found to be predictors of the concentration of glycated nail proteins. Glycation of nail proteins takes place in the deep layer of finger nails, which is in close contact with blood vessels and interstitial fluid. Glycation of nail proteins can be regarded as a representative marker for diabetic glycation-associated target organ damage.
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DOI：
10.1016/j.cca.2013.07.013
发表时间：
2013-10-21
期刊：
Clinica chimica acta; international journal of clinical chemistry
影响因子：
--
作者：
Anguizola J;Matsuda R;Barnaby OS;Hoy KS;Wa C;DeBolt E;Koke M;Hage DS
通讯作者：
Hage DS
DOI：
10.1007/bf02872403
发表时间：
2004-01-01
期刊：
Indian journal of clinical biochemistry : IJCB
影响因子：
--
作者：
Jansirani;Anathanaryanan, P H
通讯作者：
Anathanaryanan, P H
影响因子：
2.7
作者：
de Berker, David
通讯作者：
de Berker, David
影响因子：
4.6
作者：
KITAHARA T;OGAWA H
通讯作者：
OGAWA H
DOI：
10.1111/j.1467-2494.2007.00372.x
发表时间：
2007-08-01
影响因子：
2.3
作者：
de Berker, D. A. R.;Andre, J.;Baran, R.
通讯作者：
Baran, R.