Correlation of nasopharyngeal cultures prior to and at onset of acute otitis media with middle ear fluid cultures.
Correlation of nasopharyngeal cultures prior to and at onset of acute otitis media with middle ear fluid cultures.
复制标题
DOI：
10.1186/s12879-014-0640-y
复制
发表时间：
2014-12-05
影响因子：
3.7
通讯作者：
Pichichero ME
中科院分区：
文献类型：
--
作者：
Kaur R;Czup K;Casey JR;Pichichero ME
我们试图确定在健康访问或急性中耳炎(AOM)发作时采集的鼻咽(NP)培养是否可以预测通过鼓室穿刺术获得的中耳液(MEF)培养所确定的中耳分离株的耳病原体混合和抗生素敏感性。在2006年6月至2013年8月对619名儿童进行的7年前瞻性研究中，6-30个月大的儿童在健康访问时获得NP培养，在AOM发作时获得NP和MEF(通过鼓室穿刺术)的培养。共收集2601份NP和530份MEF样本。在健康访问期间，从656株(31.7%)NP培养物中分离到肺炎链球菌(Spn)，而从非分型流感嗜血杆菌(NTHi)和卡他莫拉氏菌(Mcat)中分离出253株(12.2%)和723株(34.9%)。在AOM发病时，530份NP标本中有256份(48.3%)培养出Spn阳性，223份(42%)培养出NTHi，251份(47.4%)培养出Mcat，无论是单独培养还是联合培养。在530次AOM就诊中，152例(28.7%)MEF患者中分离出Spn，而NTHi患者中分离到196例(37.0%)，Mcat患者中分离出104例(19.6%)Spn。在AOM发病时收集的NP培养，而不是在儿童健康时收集的NP培养，对流行病学抗生素敏感性模式评估具有预测价值。以MEF培养为金标准，AOM发病时的NP培养与耳病病原体混合的相关性比健康就诊时的NP培养更密切，但相关性太低，不能推荐作为MEF培养的替代品。出于流行病学的目的，当在AOM开始时采集样本时，可以通过NP培养结果来预测MEF分离株的抗生素敏感性。本文的在线版本(doi：10.1186/s12879-0140640-y)包含补充材料，授权用户可以使用。
We sought to determine if nasopharyngeal (NP) cultures taken at times of healthy visits or at onset of acute otitis media (AOM) could predict the otopathogen mix and antibiotic-susceptibility of middle ear isolates as determined by middle ear fluid (MEF) cultures obtained by tympanocentesis. During a 7-year-prospective study of 619 children from Jun 2006-Aug 2013, NP cultures were obtained from 6-30 month olds at healthy visits and NP and MEF (by tympanocentesis) at onset of AOM episodes. 2601 NP and 530 MEF samples were collected. During healthy visits, S. pneumoniae (Spn) was isolated from 656 (31.7%) NP cultures compared to 253 (12.2%) for Nontypeable Haemophilus influenzae (NTHi) and 723 (34.9%) for Moraxella catarrhalis (Mcat). At onset of AOM 256 (48.3%) of 530 NP samples were culture positive for Spn, 223 (42%) for NTHi and 251 (47.4%) for Mcat, alone or in combinations. At 530 AOM visits, Spn was isolated from 152 (28.7%) of MEF compared to 196 (37.0%) for NTHi and 104 (19.6%) for Mcat. NP cultures collected at onset of AOM but not when children were healthy had predictive value for epidemiologic antibiotic susceptibility pattern assessments. NP cultures at onset of AOM more closely correlate with otopathogen mix than NP cultures at healthy visits using MEF culture as the gold standard, but the correlation was too low to allow NP cultures to be recommended as a substitute for MEF culture. For epidemiology purposes, antibiotic susceptibility of MEF isolates can be predicted by NP culture results when samples are collected at onset of AOM. The online version of this article (doi:10.1186/s12879-014-0640-y) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.6
作者：
Casey, Janet R.;Kaur, Ravinder;Pichichero, Michael E.
通讯作者：
Pichichero, Michael E.
影响因子：
3.6
作者：
Arguedas, Adriano;Dagan, Ron;Noel, Gary J.
通讯作者：
Noel, Gary J.
影响因子：
3.6
作者：
Block, SL;McCarty, JM;Tack, KJ
通讯作者：
Tack, KJ
影响因子：
13.6
作者：
Labout, Joost A. M.;Duijts, Liesbeth;Lebon, Ankie;de Groot, Ronald;Hofman, Albert;Jaddoe, Vincent V. W.;Verbrugh, Henri A.;Hermans, Peter W. M.;Moll, Henriette A.
通讯作者：
Moll, Henriette A.
影响因子：
4.2
作者：
Friedel, V.;Zilora, S.;Pichichero, M. E.
通讯作者：
Pichichero, M. E.